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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guildeline study with acceptable restrictions.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
-Melting point: 63°C
-Boiling point: 205°C
-Purity: 99.0 %
-Lot number: 08826KS
-storage conditions: room temperature, light shielding
-Test material was analyzed and checked using Infrared spectroscopy (for example to monitor stability and storage conditions)
Analytical monitoring:
yes
Remarks:
Measurement of test substance concentration of test solution using HPLC and UV detection at 230 nm.
Details on sampling:
10 mg of the test substance was weighted, added to 100 mL water and stirred for about 24 hours (light shielding) to prepare a stock solution containing 100 mg/L.
The test solution was measured at the beginning (0 h) and at the end (48 h) of the experiment.
5 mL acetonitrile and 5 mL pure water were added to 10 mL of each analysis sample and measrued at HPLC.
Vehicle:
no
Details on test solutions:
Preparation of test solution
10 mg of the test substance was weighed, added to 100 mL of dilution water and stirred for about 24 hours. A stock solution containing 100 mg/L was prepared and was added to dilution water.
100 mL was dispensed into 4 test vessels per concentration.
Dilution water without adding test substance was used in the control group.
Preparation of the test solution was carried out immediately before Daphnia was added.
Test organisms (species):
Daphnia magna
Details on test organisms:
Juveniles of Daphnia magna were used for testing within 24 hours after birth. This specie was obtained from the National Institute for Environmental Studies by breeding at the test facility. Every 6 months the acute swimming inhibition test was conducted using reference substance potassium dichromate, and it was confirmed that there was no abnormality in the sensitivity of Daphnia.
Test type:
static
Water media type:
freshwater
Remarks:
Dechlorinated water (Tap water treated with activated carbon, residual chlorine etc. removed, sufficiently ventilated)
Limit test:
no
Total exposure duration:
48 h
Hardness:
23.0 mg/L
Test temperature:
20.5 °C +/- 1.0°C
pH:
7.5 - 7.7
Dissolved oxygen:
minimum dissolved oxygen concentration was 8.8 mg/L throughout the trial period
Nominal and measured concentrations:
nominal concentration of test substance: 0.08, 0.11, 0.14, 0.18, 0.23, 0.30 mg/L;
geometric mean of measured concentrations of test subsance: 0.054, 0.088, 0.118, 0.166, 0.209, 0.290 mg/L
Results of analysis of the test substance concentration (at 0 and 48 h) showed that the substance stayed stable through the test duration.
Details on test conditions:
Experimental period: 48 hours;
Number of stations: 4 stations per concentration zone;
Number of organisms: 20 heads / 1 concentration group (5 heads per row and 20 concentration sections per row);
Test container: 200 mL glass beaker;
Test solution volume: 100 mL per container (1 strand);
Temperature: 20.0;
Lighting: 24 hours dark;
Feeding: No feeding
6 concentrations and control
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.167 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: via Probit method
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.088 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
other: Lowest concentration in 100% immobility
Effect conc.:
0.29 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.257 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: via Probit method
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
0.118 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: Lowest concentration in 100% immobility
Effect conc.:
> 0.29 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No effect could be seen in the control confirming the validity of the test.
Reported statistics and error estimates:
95% confidence interval: 0.151 - 0.185 mg/L (Probit method)

Immobility of Daphnia magna

 Nominal concentration  Measured concentration Cumulative number of immobility (Percent immobility)    
 (mg/L)  (mg/L)  24 -Hours  48 -Hours
 0.08 0.054  0 (0)  0 (0) 
 0.11 0.088  0 (0)  0 (0) 
 0.14 0.118  0 (0)  0 (0) 
 0.18 0.166  2 (10)  8 (40) 
 0.23 0.290  5 (25)  16 (80) 
 0 30 0.290  13 (65)  20 (100) 
Validity criteria fulfilled:
yes
Remarks:
In the control not more than 10 % of the daphnids should have been immobilised. The dissolved oxygen concentration at the end of the test should be >= 3 mg/L in control and test vessels
Conclusions:
The acute toxicity of 1,8-napthylenediamine invertebrates (Daphnia magna) was determined during 48 hours and showed an EC50 of 0.167 mg/L and a NOEC of 0.088 mg/L.
Executive summary:

The test has been conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under static conditions for 48 h. Groups of 20 animals were exposed to a range of concentrations (0.08, 0.11, 0.14, 0.18, 0.23, 0.3 mg/L) of 1,8-naphthylenediamine in dilution water. The number of swimming inhibiton of Daphnia was observed 24 and 48 h after the start of exposure. EC50 was calculated by Probit method. The NOEC was defined as the highest concentration range where no swimming inhibition was observed. EC50 = 0.167 mg/L and NOEC = 0.088 mg/L could be determined for 48 h.

The study is classified as acceptable and satisfies the guideline requirements for the toxicity study to Daphnia.

Description of key information

The test has been conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under static conditions for 48 h. Groups of 20 animals were exposed to a range of concentrations (0.08, 0.11, 0.14, 0.18, 0.23, 0.3 mg/L) of 1,8-naphthylenediamine in dilution water. The number of swimming inhibiton of Daphnia was observed 24 and 48 h after the start of exposure. EC50 was calculated by Probit method. The NOEC was defined as the highest concentration range where no swimming inhibition was observed. EC50 = 0.167 mg/L and NOEC = 0.088 mg/L could be determined for 48 h.

The study is classified as acceptable and satisfies the guideline requirements for the toxicity study to Daphnia.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.167 mg/L

Additional information