4'-sulphamoylacetanilide

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from Danish QSAR database.

Justification for type of information:

Data is from Danish QSAR database.

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Determination of estimated 72 hrs EC50 value of test substance on Pseudokirchneriella subcapitata by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database. It is a continuous (Q)SAR model based on structural features and numeric molecular descriptors.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: N-(4-sulfamoylphenyl)acetamide
- Molecular formula: C8H10N2O3S
- Molecular weight: 214.244 g/mol
- Smiles notation: c1(ccc(NC(C)=O)cc1)S(N)(=O)=O
- InChl: 1S/C8H10N2O3S/c1-6(11)10-7-2-4-8(5-3-7)14(9,12)13/h2-5H,1H3,(H,10,11)(H2,9,12,13)
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Selenastrum Capricornutum

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

Estimated data

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

No data available

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

No data available

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

161.75 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Domain applicability - Battery : IN

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Table: Table showing the Pseudokirchneriella subcapitata 72 hr EC50 values estimated by two different models i.e, Leadscope and SciQSAR & the average EC50 value was given by the third model i.e, Battery model.

DK	Battery	Leadscope	SciQSAR
Pseudokirchneriella subcapitata 72 hr EC50 (mg/l)
Domain	IN

 

Where,

IN = inside applicability domain

Validity criteria fulfilled:

not specified

Conclusions:

Based on inhibition in growth rate of Pseudokirchneriella subcapitata, the estimated 72 hrs EC50 value by three different model i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database was evaluated to be 161.75 mg/l.

Executive summary:

Based on the prediction done using thethree different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substance N-(4-sulfamoylphenyl)acetamide(121 -61 -9). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance  N-(4-sulfamoylphenyl)acetamide is estimated to be 161.75mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemical N-(4-sulfamoylphenyl)acetamide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.

Description of key information

Based on the prediction done using the three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substance N-(4-sulfamoylphenyl)acetamide(121 -61 -9). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance  N-(4-sulfamoylphenyl)acetamide is estimated to be 161.75mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemical N-(4-sulfamoylphenyl)acetamide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

161.75 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of N-(4-sulfamoylphenyl) acetamide (121-61-9),and RA chemical 4-acetamidobenzenesulfonyl chloride (121-60-8), N-(4-hydroxyphenyl)acetamide (103-90-2) on the growth of green algae. The studies are as mentioned below:

 

 

The first weight of evidence study was from Danish QSAR, 2017 for the target chemical, N-(4-sulfamoylphenyl) acetamide. Based on the prediction done using the three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substance N-(4-sulfamoylphenyl)acetamide(121 -61 -9). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance  N-(4-sulfamoylphenyl)acetamide is estimated to be 161.75mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemical N-(4-sulfamoylphenyl)acetamide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not-classified as per the CLP classification criteria.

 

In the second weight of evidence study from QSAR, 2017 for the target chemical, N-(4-sulfamoylphenyl) acetamide. Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for N-(4-sulfamoylphenyl) acetamide (121-61-9). The EC50 value was estimated to be 156.28 mg/l when N-(4-sulfamoylphenyl) acetamide was exposed toon Desmodesmus subspicatus for 72 hrs.

 

Similarly another weight of evidence study for N-(4-sulfamoylphenyl) acetamide was taken from ECOSAR, 2017. Based on the prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance N-(4-sulfamoylphenyl) acetamide (121 -61 -9). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 1360mg/l for green algae for 96hrs duration. Based on this value, it can be concluded that the test chemical N-(4-sulfamoylphenyl)acetamide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Similarly the effect of test substance N-acetylsulphanilyl chloride, CAS No. (121-60-8), Lab report Sustainable support service was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25, 12.5, 25, 50, 100 and 200 mg/L were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated from equation and graphically through probit analysis was observed to be193.43 mg/L and 200 mg/L respectively. Thus chemical was consider as nontoxic to aquatic algae and not classified as per the CLP classification criteria.

 

The effect of test item paracetamol, CAS No. 103-90-2 which was the RA chemical from UERL lab was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 112.666mg/l. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Paracetamol does not exhibit toxicity to aquatic algae (Chlorella vulgaris).

 

Similarly in sixth weight of evidence study for N-(4-hydroxyphenyl) acetamide (103-90-2) J-check, 2017, Short term toxicity study of Acetaminophen on the growth of freshwater green algae was carried out for 72 hrs. The effective concentration (EC50) value of N-(4-Hydroxyphenyl) acetamide (Paracetamol) in aquatic green algae in a 72 hr study on the basis of growth rate effect and AUG was > 460 mg/l and 150 mg/l. But the NOEC on the basis of AUG and growth rate was 22 mg/l and 46 mg/l. Based on the growth rate and AUG of green algae by considering EC50 (>460 mg/l), N-(4-Hydroxyphenyl) acetamide was nontoxic and cannot be classified as toxic to aquatic algae as per the CLP classification criteria.

 

Based on the data obtain from various sources for target and RA chemicals, it can be concluded that the substance N-(4-sulfamoylphenyl) acetamide (121-61-9) is considered to be not toxic to algae and cannot be classified toxic as per the criteria mentioned in CLP regulation.