Ethyldimethylamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29.02.1988 - 02.03.1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study, Non-GLP

Qualifier:

according to guideline

Guideline:

other: EU Directive 79/831/EEC, Annex V, part C

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Preparation of test media:
The test medium of the highest test concentration, 200 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: Daphnia magna Straus
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

ACCLIMATION
Total water hardness: 2.70 +/- 0.5 mmol/l
Acidity constant - pH 4.3: 0.80 +/- 0.1 mmol/l
Molar ratio: Ca:Mg ca. 4 :1
Oxygen content > 2 mg/l
Light conditions: 16 : 8 hours day-night regime, diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length
- Type and amount of food: : Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.70 +/- 0.5 mmol/L

Test temperature:

18-20 °C

pH:

7.7-8.3

Dissolved oxygen:

9.05 - 8.6 mg/L

Nominal and measured concentrations:

Nominal concentrations:6.25, 12.5, 25, 25, 50, 100, 200 mg/L. Additionally, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: : according to the test guideline DIN 38412, L 11
pretreatment steps: tap water; 6 µm- and charcoal-filtration; H2SO4 was added to reduce alkalinity up to pH 4.3; distilled water was added to reduce water-hardness; water was aerated (oil-free air) unil saturated with oxygen; water was stored for at least 24 h for stabilization.
- Ca/mg ratio: 4:1
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours day-night regime
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Visually after 0, 3, 6, 24, 48 h

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

not neutralized

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

39.23 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

not neutralized

Remarks on result:

other: 32.31 - 47.63

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

not neutralized

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

not neutralized

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

57.43 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

not neutralized

Remarks on result:

other: 49.79 - 66. 25

Number of mobile test animals after exposure (x h) to various test concentrations:

Concentration (mg/L)	0 h	3 h	6 h	24 h	48 h
0	20	20	20	20	20
6.25	20	20	20	20	20
12.5	20	20	20	20	20
25	20	20	20	20	16
50	20	20	20	14	7
100	20	5	0	0	0
200	20	0	0	0	0

At 100 and 200 mg/L toxic effect may be due to the high pH in the test vessels (up to pH 10.57)
 

pH values after

concentration (mg/L)	0 h	48 h
0	8.07	8.0
6.25	8.89	8.23
12.5	9.25	8.26
25	9.61	8.39
50	9.95	8.81
100	10.27	9.36
200	10.57	9.45

Executive summary:

Acute toxicity to Daphnia magna has been investigated according to the methods outlined by EU Directive 79/831/EEC, Annex V, part C.

Daphnia magna were exposed for 48 hours to an aqueous solution of ethyldimethylamine at nominal concentrations of 6.25 - 12.5 - 25 - 50 - 100 and 200 mg/L.

EC 50 - 48h determined is of 39.23 mg/L and NOEC is of 12.5 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

39.23 mg/L

Additional information

Ethyldimethylamine toxicity to daphnids has been investigated by BASF (1988). The methodology followed is EU Directive 79/831/EEC, Annex V, part C. with exposure duration of 48 h. In this context, the 48 h EC50 determined is of 39.23 mg/L and the NOEC of 12.5 mg/L.