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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Study period:
Feb 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well reported GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): hydrocortisone-21-acetate (ZK 5192)
- Analytical purity: 100.2%´
- Lot/batch No.: 10035003
- Expiration date of the lot/batch: Feb 2003

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 6.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

2 daphnids (10%) were immobilized between 24 and 48 hours. The test concentration was determined by TOC analysis with 6.2 mg/L. Thus the EC50/48 h was higher than a saturated solution of hydrocortison acetate with 6.2 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Since acc. to guideline 10% immobilisation is accepted in control groups the immobilisation observed is considered to be spontanous rather than substance-related.
Executive summary:

The aquatic toxicity of the test compound was studied in an acute immobilization test with Daphnia magna (limit test). The daphnids were exposed to a saturated solution of the test compound for 48 hours. 2 daphnids (10%) were immobilized between 24 and 48 hours. This was assumed to be spontaneous, since up to 10% immobilized daphnids are accepted for the control group.The test concentration was determined by TOC analysis with 6.2 mg/L. Thus the EC50/48 h was higher than a saturated solution of hydrocortison acetate with 6.2 mg/L.