Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexachloroplatinic acid
EC Number:
241-010-7
EC Name:
Hexachloroplatinic acid
Cas Number:
16941-12-1
Molecular formula:
Cl6Pt.2H
IUPAC Name:
Hexachloroplatinic acid
Test material form:
liquid
Details on test material:
- Colour/form: orange liquid
- Density: 2.069 g/mL

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Duplicate samples of the control and of test concentrations were collated at the beginning and at the end of the test. Samples were taken from the approximate centre of the aquaria without mixing of the test media. Prior to analysis, samples were stored in a refrigerator.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution of the highest test concentration of nominal 1000 mg/L was prepared by dissolving 300 mg test item into 3000 mL test water by intense stirring for 10 min. Adequate volumes of test medium were then diluted with test water to prepare test media.
- Controls: test medium without test item

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenzuchtbetrieb Tautenhahn, 98646 Trostadt, Germany
- Age at study initiation: juveniles
- Length at study initiation (length definition, mean, range and SD): mean body length 4.5 ± 0.21 cm (n = 10)
- Weight at study initiation (mean and range, SD): mean body weight 1.15 ± 0.18 g (n = 10)

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: commercial fish diet
- Health during acclimation (any mortality observed): 4 fish died (< 5 %)

FEEDING DURING TEST: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None

Test conditions

Hardness:
250 mg/L as CaCO3
Test temperature:
14 °C
pH:
7.8 - 8
Dissolved oxygen:
10.2 - 12.6 mg/L
Nominal and measured concentrations:
Nominal concentrations: 100, 46, 21, 10, 4.6 mg test item/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 20-L glass aquaria filled with 16 L test medium
- Aeration: slightly aerated throughout the test
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Total Hardness: 250 mg/L as CaCO3
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1 (based on molarity)
- Conductivity: < 5 μS/cm
- Intervals of water quality measurement: daily measurements

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 hour dark
- Light intensity: 200 - 1200 lux

EFFECT PARAMETERS MEASURED: Fish were observed every 24 hours for symptoms of intoxication and mortality.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: about 2.1
- Range finding study
- Test concentrations: Not reported
- Results used to determine the conditions for the definitive study: the concentrations of the test item in the test medium were found to be constant throughout the test
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
76.55 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % Confidence Intervals: 53.89 - 108.75 mg test item/L
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
25.78 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % Confidence Interval: 18.15 - 36.63 mg Pt/L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
21 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
7.07 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
15.49 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mortality (fish)
Details on results:
In the control and at the test concentration up to 21 mg test item/L all fish survived until the end of the test. After 96 hours of exposure, one and five fish died at 46 and at 100 mg test item/L, respectively. After 96 hours of exposure at 100 mg test item/L, strong ventilation was observed in the two remaining fish.
Reported statistics and error estimates:
The LC50 and 95% Confidence Intervals were calculated by Probit analysis interpolation. The NOEC and LOEC values were directly determined from the raw data.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1. Mortality and symptoms of toxicity of the test item to Oncorhyncus mykiss at 0, 24, 48, 72 and 96 hours of exposure. The reported values in the table represent the number of dead fish/number of fish with intoxication symptoms (dead fish are added to the sum of fish with symptoms)

 Nominal concentration (mg test item/L)  0 h 24 h  48 h 72 h  96 h 
 Control  0/0 0/0  0/0  0/0  0/0 
 4.6 0/0 0/0  0/0  0/0  0/0 
 10 0/0  0/0  0/0  0/0  0/0 
 21 0/0  0/0  0/0  0/0  0/0 
 46 0/0 0/0  0/0  0/0  1/1 
 100 0/0  1/1  1/5 (SV)  1/5 (SV)  5/7 (SV) 
 LC50 (mg test item/L)  > 100  > 100  > 100  > 100 76.55 
 95 % confidence intervals  -  -  -  -  53.89 - 108.75

SV = Strong Ventilation

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The validity criteria were fulfilled as no fish died in the control and oxygen saturation was always > 60%
Conclusions:
The 96-hour LC50 of hexachloroplatinic acid to Rainbow trout (Oncorhynchus mykiss) was determined to be 76.55 mg test item/L, corresponding to 25.78 mg Pt/L.
Executive summary:

The acute toxicity of hexachloroplatinic acid to Oncorhyncus mykiss was assessed. The study is reliable without restrictions, being GLP-compliant and having followed the standard guidelines OECD guideline 203 and EU Method C.1.

A static test procedure was followed. Five test concentrations were used and these were analysed using Graphite furnace-AAS method. As measured concentrations were above 100 % of the nominal values throughout the study, with a mean of 107 % of nominal, results were based on nominal concentrations. No protocol deviations were recorded. All validity criteria were met. The 96-hour LC50 was determined to be 76.55 mg test item/L, corresponding to 25.78 mg Pt/L.