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EC number: 252-276-9 | CAS number: 34893-92-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation
- Remarks:
- in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Principles of method if other than guideline:
- The modified local lymph node assay (IMDS) was performed on 24 female NMRI mice (6 animals/test item group and 6 control animals) with the following test item concentrations: 0 (vehicle control), 1, 3 and 10%. The modification refer to the measurement of cell proliferation by cell counting instead of radioactive labelling. In addition , the acute inflammatory skin reaction is determined to discriminate specific from non-specific activation of immune competent cells in the draning lymph nodes. The aim of these investigations was to establish whether there is any specific (sensitizing) or non-specific (irritant) stimulating potential of the test item 3.5-Dichlorphenylisocyanat.
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 1,3-dichloro-5-isocyanatobenzene
- EC Number:
- 252-276-9
- EC Name:
- 1,3-dichloro-5-isocyanatobenzene
- Cas Number:
- 34893-92-0
- Molecular formula:
- C7H3Cl2NO
- IUPAC Name:
- 1,3-dichloro-5-isocyanatobenzene
- Details on test material:
- content: 99.9%
solid, white powder
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- female
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 0, 1, 3, or 10%
- No. of animals per dose:
- 6 animals/test item group and 6 control animals
Results and discussion
In vivo (LLNA)
Results
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- Tabular summary: Direct LLNA (NMRI mice, female, 6 animals/group) dose (%) = 0 weight index , cell count index 0 1.00 1.00 1 2.47 2.61 3 3.28 3.36 10 4.09 3.88 The NMRI mice showed a clear increase in the weights of the draining lymph nodes and in the stimulation indices for cell counts in all dose groups, which are of statistical significance, compared to control animals after application of the test item 3,5 -Dichlorphenylisocyanat. The positive level, which is 1.4 for cell count indices, has been exceeded in all dose groups
Any other information on results incl. tables
The positive level for ear swelling which is 2x10E-2 mm increase, i.e. about 10% of the control values, has just been exceeded in the high dose group. Statistically significant increases of the ear swelling were determined for the mid and the high dose group animals compared to the vehicle treated animals. A statistically significant increase of the ear weight was determined for the high dose group animals compared to vehicle treated animals.
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
- Executive summary:
The modified local lymph node assay (IMDS) was performed on 24 female NMRI mice (6 animals/test item group and 6 control animals) with the following test item concentrations: 0 (vehicle control), 1, 3 and 10%. The modification refer to the measurement of cell proliferation by cell counting instead of radioactive labelling. In addition , the acute inflammatory skin reaction is determined to discriminate specific from non-specific activation of immune competent cells in the draning lymph nodes. The aim of these investigations was to establish whether there is any specific (sensitizing) or non-specific (irritant) stimulating potential of the test item 3.5-Dichlorphenylisocyanat.
Beside a non-specific irritant property of the test item, a specific activation of the cells of the immune system via dermal route was determined after application of 1, 3, and 10% 3,5 -Dichlorphenylisocyanat by the method used. Thus, 3,5 -Dichlorphenylisocyanat has to be classified as at least a strong skin sensitizer.
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