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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Principles of method if other than guideline:
Study conducted according to Bringmann and Kuehn
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-aminobenzenesulphonic acid
EC Number:
204-473-6
EC Name:
3-aminobenzenesulphonic acid
Cas Number:
121-47-1
Molecular formula:
C6H7NO3S
IUPAC Name:
3-aminobenzene-1-sulfonic acid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Strain: DSM 50026
- Culture medium: AK-Medium according to DIN 38412 part 8 (draft)
- Temperature: 24°C
- Duration of growth: 7 +/-1 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
20°C
pH:
3.4 - 6.3
Nominal and measured concentrations:
- Concentrations tested (nominal): 7.81, 15.625, 31.25, 62.5, 125, 250, 500 and 1000 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
156.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
199.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
242.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

- Decrease of pH-value at test concentrations > 125 mg/L:

Conc. (mg/L) pH

7.81 6.3

15.625 6.2

31.25 6.3

62.5 6.1

125 6.0

250 4.3

500 3.8

1000 3.4

Applicant's summary and conclusion