Registration Dossier
Registration Dossier
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EC number: 204-473-6 | CAS number: 121-47-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.08.1976 - 23.09.1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-aminobenzenesulphonic acid
- EC Number:
- 204-473-6
- EC Name:
- 3-aminobenzenesulphonic acid
- Cas Number:
- 121-47-1
- Molecular formula:
- C6H7NO3S
- IUPAC Name:
- 3-aminobenzene-1-sulfonic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Details on inoculum:
- The details on the type and amount of inoculum have not been documented. However, the study director, Dr. H. Wellens was particitpant in the laboratory comparison tests for the development of the OECD test methods 301 and 302. For this reason it is most likely that the test conditions were already similar to the later OECD guideline 301E.
- Duration of test (contact time):
- 32 d
Initial test substance concentration
- Initial conc.:
- 48 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST SYSTEM
- Test vessel: 2 L Erlenmeyer flasks
- Treatment of test samples: filtrated through membrane filters, nominal diameter of the pores 0.2 µm
- Incubation of test vessels: testing was conducted on a rotary shaker with a diameter of the shaking circle of 5 cm and exposed to room temperature (20 - 22°C). The shaking frequency was set to about 70 - 100 rpm
Reference substance
- Reference substance:
- other: D(+)-glucose (monohydrate)
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 38
- Sampling time:
- 26 d
- Details on results:
- - Degradation kinetics:
Time [d] Degradation [%]
1 0
2 26
12 0
16 18
26 38
30 42
BOD5 / COD results
- Results with reference substance:
- - Degradation (DOC-removal) of test substance after 12 days: 79 %
Applicant's summary and conclusion
- Interpretation of results:
- other: partly/moderately biodegradable
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