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EC number: 807-040-5
CAS number: 4538-42-5
A series of standard solutions with concentration at 5.0, I 0, 30, 50
and 100 were measured
under the TOC conditions mentioned above. Based on the test result, a
equation was obtained with the peak area vs. standard solution
concentration, A =798c -
1279, with good linearity of r2 = 0.9984, where A represents
peak area (AU), and c is TOC
concentration (mg/L). The results show that linearity for concentration
range of 5.0 mg/L
to 100 mg/Lis good
The inherent biodegradation test on 1,5-Diisocyanatopentan (PDI) was
performed according to "The guidelines for the testing of chemicals"
SEPA(HJ/T 153 -2004), "The guidelines for the testing of chemicals,
Degradation and Accumulation" (the 2nd edition) (Beijing: China
Environment Press. 2013), and Procedure 302C of the 'Guidelines for
Testing of Chemicals' of the OECD: "Inherent Biodegradability: Modified
MITI Test (II)" (1981). Test solutions were prepared in an inorganic
salts medium, inoculated with a number of micro-organisms collected from
10 places in Nanjing city. During the test, the temperature was kept at
(25±2) °C. The test was valid because the level of biodegradation of the
reference substance aniline was 76.2% after 7 days (> 40%), and 88.5 %
after 14 days (>65% ), and the recovery rate of residual amount of the
test compound in the "abiotic control" is found to be more than 10%
after 28 days.
The BOD results showed that inherent biodegradation of the test
substance (POI) was 8.61 % after 28 days.
The TOC results showed that inherent biodegradation of the test
substance (POI) was 2.58 % after 28 days.
According to OECD Guidelin 301F a
suspension of 100 mg/L PDI in a mineral medium, equalling to 50-100 mg
ThOD or COD/Litre as the nominal sole source of organic carbon, was
stirred in a 250 ml closed flask in the dark with an inoculum originally
collected from a local, predominantly municipal wastewater treatment
plant. In this assay, biodegradation was estimated by biological oxygen
demand (BOD) over time. BOD was measured daily by a manometer. The
incubation temperature was (22 ± 1 °C), ph 7.2 -8.0. The concentration
of innoculum was 30 mg /L suspended solids. The endogenous activity of
the inoculum was checked running parallel blanks with inoculum but
without test item. A reference compound (sodium benzoate) was run in
parallel to check the operation of the procedures. A toxicity control
(test item and reference compound mixed, one replicate) was run in
parallel, to ensure that the chosen concentration of the test item was
not inhibitory to microorganisms. Because of the nature of
biodegradation and of the mixed bacterial populations used as inoculum,
determinations of test item and inoculum blank were carried out in
triplicate and of reference compound in duplicate. The test item is an
N-containing substance. Therefore, the concentration of nitrite and
nitrate was determined at the start of the test. The oxygen consumed by
nitrification was not determined after 28 days, because low degradation
of the test item was observed. Within 28 days, a degradation rate of 26
% was determined. The substance is
considered to be "Not Readily Biodegradable".
Within 28 days, a degradation rate of 26 % was determined (Spoo-Klöppel,
2016). Therefore the substance is considered to be "Not Readily
In an inherent biodegradation test a BOD result of 8.61 % after 28 days
and a TOC result of 2.58 % after 28 days was measured (Zhou, 2017). The
substance is not inherently biodegradable.
The substance is considered to be
"Not Readily Biodegradable". The substance is "Not
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