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EC number: 259-105-7
CAS number: 54326-11-3
-Direct MTT reduction: The MTT solution
containing the test item did not turn blue, indicating that test item
did not directly reduce MTT.
acceptance criteria were satisfied according to the protocol criteria
for both the positive control (relative mean viability ≤ 40% and
standard deviation ≤ 18%) and negative control (OD540≥ 0.6 and standard
deviation of individual tissue viability ≤18%). The standard deviation
of the triplicate treated tissues was 3.4 % and hence the test item
acceptance criterion (≤ 18%) was also satisfied.
Mean OD540of triplicate tissues
±SD of OD540
Relative Individual tissue viability (%)
Relative mean viability (%)
±SD of Relative mean viability (%)
Negative Control Item1
Positive Control Item1
= Standard deviation
= The mean viability of the negative control tissues is set at 100%
group shared with test laboratory project number 41202604
benzoate C16-18-fatty acids complexesis
considered to be non-irritant using the EPISKIN (TM) human epidermis
model. The skin irritation potential of the test item was evaluated
using EPISKIN (TM) reconstructed human epidermis model after a treatment
period of 15 minutes followed by a post-exposure period of 42 hours
using a colourimetric MTT reduction assay following OECD guideline 439
in an experimental proprietary study (Harlan 2013). The quality criteria
required for the acceptance of results in the test were satisfied and
the study is considered reliable and relevant for use.
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