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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authoritative databases
Qualifier:
according to
Guideline:
other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
Principles of method if other than guideline:
Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.
GLP compliance:
not specified
Radiolabelling:
not specified
Vehicle:
not specified
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Length at study initiation (lenght definition, mean, range and SD): 8
- Sex: MF
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk
Test temperature:
25°C
pH:
6.0-8.5
Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations : TLm(48h) ≥ 50 ppm (w/v), Rice fish (Oryzias latipes
Nominal and measured concentrations:
Nominal concentrations:
1st concentration area: 0.3 mg/l (350 ppm(w/v))
2nd concentration area: 0.03 mg/l (35.0 ppm(w/v))
Reference substance (positive control):
not specified
Lipid content:
2.8 %
Remarks on result:
other: Other details not known
Conc. / dose:
0.3 mg/L
Type:
BCF
Value:
<= 0.7 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: Other details not known
Conc. / dose:
0.03 mg/L
Type:
BCF
Value:
<= 7.1 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: Other details not known

Bioaccumulation test results:

 

 

2 W

3 W

4 W

6 W

1st Concentration area

 

Test substance concentration (ppm (w/v))

0.280

0.276

0.275

0.270

Bioconcentration factor (L/kg)  

≤ 0.7

≤ 0.7

≤ 0.7

≤ 0.7

≤ 0.7

≤ 0.7

≤ 0.7

≤ 0.7

2nd Concentration area

 

Test substance concentration (ppm (w/v))

0.0265

0.0268

0.0269

0.0265

Bioconcentration factor (L/kg)  

≤ 7.1

≤ 7.1

≤ 7.1

≤ 7.1

≤ 7.1

≤ 7.1

≤ 7.1

≤ 7.1

Validity criteria fulfilled:
not specified
Conclusions:
The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively.
Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" under flow through conditions at a temperature of 25°C and pH range 6.0-8.5, respectively. Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.3mg/l and 0.03 mg/l, respectively. Analytical method involve therecovery ratio:Test water : 1st concentration area : 94.3 %, 2nd concentration area : 98.6 %, Fish : 88.5 %, - Limit of detection : Fish : 0.188 ppm. Range finding study involve theTLm(48h) ≥ 50 ppm (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism was determined to be 2.8%.The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

Description of key information

Bioaccumulation study was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (authoritative database, 2018). The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" under flow through conditions at a temperature of 25°C and pH range 6.0-8.5, respectively. Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.3mg/l and 0.03 mg/l, respectively. Analytical method involve therecovery ratio:Test water : 1st concentration area : 94.3 %, 2nd concentration area : 98.6 %, Fish : 88.5 %, - Limit of detection : Fish : 0.188 ppm. Range finding study involve theTLm(48h) ≥ 50 ppm (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism was determined to be 2.8%.The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

Key value for chemical safety assessment

BCF (aquatic species):
7.1 L/kg ww

Additional information

Various experimental key and supporting studies of the test chemical were reviewed for the bioaccumulation end point which are summarized as below:

 

In an experimental key study from authoritative database (2018), bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances" under flow through conditions at a temperature of 25°C and pH range 6.0-8.5, respectively. Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study was 0.3mg/l and 0.03 mg/l, respectively. Analytical method involve therecovery ratio: Test water : 1st concentration area : 94.3 %, 2nd concentration area : 98.6 %, Fish : 88.5 %, - Limit of detection : Fish : 0.188 ppm. Range finding study involve the TLm(48h) ≥ 50 ppm (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism was determined to be 2.8%.The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.7 L/Kg at a conc. of 0.3 mg/l and ≤ 7.1 L/Kg at a conc. of 0.03 mg/l, respectively.

 

Another bioaccumulation study was conducted for estimating the BCF (bioaccumulation factor) value of test chemical (HSDB and PubChem, 2017). The bioaccumulation factor (BCF) value was calculated using a logKow of 1.83 and a regression-derived equation. The estimated BCF (bioaccumulation factor) value of test chemical was determined to be 3.2 dimensionless.

 

In aprediction done using theKOCWIN Programof Estimation Programs Interface was used to predict the bioconcentration factor (BCF) of test chemical. The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C).

 

Using Bio-concentration Factor (v12.1.0.50374) module Bio-concentration Factor of the test chemical was estimated to be 14.3, 13.8, 10.7 and 3.33 dimensionless at range pH range 0-1, 2, 3, 4  and 5-14, respectively (ACD (Advanced Chemistry Development)/I-Lab predictive module, 2017)).

 

On the basis of above results for test chemical(from authoritative and modelling databases, 2017), it can be concluded that the BCF value of test chemicalranges from0.7to14.3,which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.