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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008-10-06 - 2009-01-29
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
Concentrations of the active ingredient N-[3(5)-Methyl-1H-pyrazol-1-yl-methyl]acetamide in the test solutions at the start of the test (0 h) and at the end of the test (96 h) were analysed.The test concentrations for P 70/05/S-4 remained within a range of 100 to 101 % of the nominal value at the start (0 h) and at the end (96 h) of the experiment. The test concentrations were found within ± 20 % of nominal throughout the test. Therefore, all results are based on the nominal concentrations.
Vehicle:
yes
Details on test solutions:
The test item is soluble in water. Therefore, a stock solution was prepared by dissolving the test item in dilution water.
The stock solution was prepared in the following way:
- Weighing of 1000.0 mg P 70/05/S-4
- Filling with dilution water up to 1 L (= stock solution A)
The test item solution was prepared by mixing the stock solution with the dilution water directly in the test vessel immediately before the transfer of the fish in the test vessels.
The control vessel contained test medium {dilution water) only.
Five days before the start of the test, the dilution water was prepared and aerated for one day in the same cabinet and at the same temperatures as the fish test was performed.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Origin of the test organism: purchased from Zierfisch-Center SCALAR Paul-Geifiler-Str. 14, 04808 Wurzen, Germany
Origin of the fish used in the test: holding and breeding in the test facility under standardised laboratory conditions
Recommended total length of the test fish: 2.0 ± 1.0 cm
Mean length of the test fish: 2.7 ± 0.3 cm
Loading: 0.4 g fish/l



Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
The sum of calcium and magnesium ions in the dilution water was 2.5 mmol/L. The proportion of Ca to Mg ions was 4:1 and of Nato K ions 10:1. The acid capacity Ka4.3of the solution was 0.8 mmol/L. Derived from the CaCI2 addition, the hardness of water was equal to 2 mmol/L CaC03 which corresponds to a hardness of 200 mg/L CaC03 (valid: > 140 and < 250 mg/L).
Test temperature:
25.8-25.9 °C

pH:
7.78
Dissolved oxygen:
8.56 mg/L
Nominal and measured concentrations:
Concentrations of the active ingredient N-[3(5)-Methyl-1H-pyrazol-1-yl-methyl]acetamide in the test solutions at the start of the test (0 h) and at the end of the test (96 h) were analysed.The test concentrations for P 70/05/S-4 remained within a range of 100 to 101 % of the nominal value at the start (0 h) and at the end (96 h) of the experiment. The test concentrations were found within ± 20 % of nominal throughout the test. Therefore, all results are based on the nominal concentrations.
Details on test conditions:
Test vessel (pool): stainless steel container (approx. 25 L volume)
Test volume: 10 l
Number of fish/vessel (= replicate): 10
Number of replicates/treatment: 1
Test medium: dilution water: reconstituted water according to ISO 6341, prepared 5 days in advance, using deionised water conductivity of deionised water: ≤ 10 µ S/cm (measured 0.630 (µS/cm)
Test procedure: static test, no adjustment of pH, no use of vehicles,
oxygen concentration: > 60 % of the air saturation value (aeration of the test vessels), no feeding during the test period
Light-dark-cycle: 16 h light: 8 h dark, daily
Reference substance (positive control):
not required
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Cumulative mortality after 24,48, 72 and 96 hours

Treatment group

P 70/05/S-4(mgIL),nominal

 

Number of dead fish

Mortality (%)

after application

control

100.0

control

100.0

24 h

0

0

0

0

48 h

0

0

0

0

72 h

0

0

0

0

96h

0

0

0

0

Effect concentration

Effect concentration

P 70/05/S-4 (mg P 70/05/S-4/L),nominal

24h 48h

72h

96 h

after

application

NOEC

100.0

100.0

100.0

100.0

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of N-[3(5)-Methyl-1H-pyrazol-1-yl-methyl]acetamide was determined according to OECD Guideline 203 (Fish, Acute Toxicity Test). Under laboratory conditions no effects of the test item on survival of Danio rerio were determined. The NOEC (no observed effect concentration) after 96 hours was determined to be 100 mg/L.
The validity criteria were accomplished:
Number of dead fish: ≤ 10 % (observed: 0 % in the control)
Dissolved oxygen concentration at the end of the test ≥ 3 mg/L in control and test vessels measured: > 7.4 mg/L)
Executive summary:
The acute toxicity of N-[3(5)-Methyl-1H-pyrazol-1-yl-methyl]acetamide was determined according to OECD Guideline 203 (Fish, Acute Toxicity Test). Under laboratory conditions no effects of the test item on survival of Danio rerio were determined. The NOEC (no observed effect concentration) after 96 hours was determined to be 100 mg/L.

The validity criteria were accomplished:

Number of dead fish:   ≤ 10 % (observed: 0 % in the control)

Dissolved oxygen concentration at the end of the test≥ 3 mg/L in control and test vessels measured: > 7.4 mg/L)

Description of key information

The acute toxicity of N-[3(5)-Methyl-1H-pyrazol-1-yl-methyl]acetamide was determined according to OECD Guideline 203 (Fish, Acute Toxicity Test). Under laboratory conditions no effects of the test item on survival of Danio rerio were determined. The NOEC (no observed effect concentration) after 96 hours was determined to be 100 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The NOEC (no observed effect concentration) after 96 hours was determined to be 100 mg/L.