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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Acute Toxicity of Some Perhalogenated Acetones
Author:
Joseph F. Borzelleca, David Lester
Year:
1965
Bibliographic source:
TOXICOLOGY AND APPLIED PHARMACOLOGY 7, 592-597 (1965)

Materials and methods

Principles of method if other than guideline:
Adult, male 1 albino rats of the Wistar strain, averaging 150 g in weight were used for these studies. They were housed 5 to a cage at a room
temperature of 23 +/- 1 °C. The animals were fasted overnight (water ad libitum but no food) prior to dosing by stomach tube. Ten rats were dosed at each of 4 or 5 dosages of a compound, After dosing, the rats were fed a commercial chow ad libitum. They were kept under observation for 4
weeks following treatment and any death occurring within that time were included in calculation of the result.
The LD50 values were calculated by the minimum approximate chi-square normit method of Berkson (1955).
GLP compliance:
not specified
Test type:
other: min. 4 doses tested
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexachloroacetone
EC Number:
204-129-5
EC Name:
Hexachloroacetone
Cas Number:
116-16-5
Molecular formula:
C3Cl6O
IUPAC Name:
hexachloropropan-2-one
Details on test material:
Boiling point: 204 °C
Specific gravity: 1.73 (25°C)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
Adult, male, albino rats of the Wistar strain, averaging 150 g in weight, were used for these studies. They were housed 5 to a cage at a room temperature of 23 +/- 1 °C. The animals were fasted overnight (water ad libitum but no food) prior to dosing by stomach tube. Ten rats were dosed at each of 4 or 5 dosages of a compound. After dosing, the rats were fed a commercial chow ad libitum. They were kept under observation for 4 weeks
following treatment and any deaths occurring within that time were included in calculation of the result.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
The animals were fasted overnight (water ad libitum but no food) prior to dosing by stomach tube. Ten rats were dosed at each of 4 or 5 dosages of a compound, After dosing, the rats were fed a commercial chow ad libitum. They were kept under observation for 4
weeks following treatment and any death occurring within that time were included in calculation of the result.
Doses:
only the result is included in the publication, no data about doses
No. of animals per sex per dose:
10 male animals per dose
Control animals:
not specified
Details on study design:
The animals were fasted overnight (water ad libitum but no food) prior to dosing by stomach tube. Ten rats were dosed at each of 4 or 5 dosages of a compound, After dosing, the rats were fed a commercial chow ad libitum. They were kept under observation for 4
weeks following treatment and any death occurring within that time were included in calculation of the result.
Statistics:
The LD50 values were calculated by the minimum approximate chi-square normit method of Berkson (1955).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
1 370 - 1 730 mg/kg bw
Based on:
test mat.
Mortality:
LD50 = 1550 +/- 180 mg/kg bw (determined with 40-50 rats)
Clinical signs:
other: Moderate to severe central nervous system depression was noted following administration, and this persisted for several days. No other signs were discernible.
Gross pathology:
No abnormalities were evident on gross autopsy.

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Remarks:
Migrated information
Conclusions:
LD50 = 1550 +/- 180 mg/kg bw (determined with 40-50 rats).
Executive summary:

The acute LD50's of several perhalogenated acetones by oral routes are presented. The compounds were administered oraJly to rats. The LD50 of hexachloroacetone after oral administration was found to be 1550 +/- 180 mg/kg bw (determined with 40-50 rats). The compound was administrated orally. hexachloroacetone caused depression of the central nervous system, but, otherwise, no specific toxic signs were associated with lethal doses. Oral administration did not lead to pulmonary damage from any of these compounds.

The mechanism of the lethal action of the latter compounds is not apparent in these studies.