Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 292-587-7 | CAS number: 90640-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Apr - 03 May 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study is complete, performed under GLP and valid. However no analysis was performed and thus the usability for risk assessment purposes is limited when assessing the bioavailable fraction.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- other: S.A.L.M. Kooyman. "Parametric analyses of mortality rates in bio-assays". Water Research 15(1981)107-119.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 25 g/l was prepared by dissolving the test substance in demineralized water containing concentrated HCl.
The presence of the test substance in the test medium caused a change of the pH, which is outside the range that can be supported by the test animal. Therefore, the pH was neutralized both in the stock solution and in the dilution water to a value between 7.0 and 7.3. The chosen test concentrations were prepared by dilution of the stock solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism was Daphnia magna; they were produced in the Akzo Research Laboratories Arnhem, dept. CRL according to NEN 6503 and Standard Operating Procedures SOP CRL T2. They were less than 24 hours old at the beginning of the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 73.1 mg Ca/l, 20.4 mg Mg/l, 14.9 °dH
- Test temperature:
- 19.7-20.8 °C
- pH:
- 7.1-7.9
- Dissolved oxygen:
- 9.0-9.2 mg/L
- Nominal and measured concentrations:
- nominal: 10, 18, 32, 56, 100 mg/L
- Details on test conditions:
- The temperature was kept between 19.7 and 20.8 °C, and the light regime was 16 hours ambient light per day.
The duration of the test was 48 hours.
The test vessels were 400 ml glass beakers, containing 250 ml of test medium. Each beaker contained 5 test animals. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 21-27.5 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A steep concentration-effect relationship was observed: no effect on mobility at 18 mg/l, and 100% immobilisation at 32 mg/l. The EC50-48 h is 24.1 mg/l. The 95% confidence limits are 21.0 and 27.5 mg/l.
The quality criteria of the test have been fulfilled: the immobilization in the control was <10%, the oxygen concentration was >2 mg/l at the end of the test, and the Daphnids in the control group have not been trapped at the surface of the water (less than 10%).
In an earlier study, the EC50-48 h of tetraethylenepentamine for Daphnia was 14 mg/l, with 95% confidence limits of 12.3 and 16.0 (Ten Berge and Blok, 1975). The (small) difference may be related to changes in the production process. - Reported statistics and error estimates:
- The EC50 was determined by an LC50 program of Griffioen (RIZA) based on a model of Kooyman (1981).
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was observed up to 18 mg/l. The EC50 was 24.1 mg/l, with 95% confidence limits of 21.0 and 27.5 mg/l (Nominal). The quality criteria of the test have been fulfilled: the immobilization in the control was <10%, the oxygen concentration was >2 mg/l at the end of the test, and the Daphnids in the control group have not been trapped at the surface of the water (less than 10%). The usability of the test reults when performing a riskassessment is limited due to the fact that only nominal concentrations are presented. This results in no information on the actuals bio-available concentration.
- Executive summary:
Tetraethylenepentamine (TEPA) was tested in an acute toxicity test with the water flea (Daphnia magna). The test was performed according to the EEC method, part C.: Methods for the determination of ecotoxicity, C.2. Acute toxicity for Daphnia. The test was carried out as a static test, in which the solution was not renewed. No mortality was observed up to 18 mg/l. The EC50 was 24.1 mg/l, with 95% confidence limits of 21.0 and 27.5 mg/l nominal. The quality criteria of the test have been fulfilled: the immobilization in the control was <10%, the oxygen concentration was >2 mg/l at the end of the test, and the Daphnids in the control group have not been trapped at the surface of the water (less than 10%).
Reference
Nominal concentration mg/L |
Cumulative number of mobile animals |
||
0 h |
24 h |
48 h |
|
Control |
60 |
60 (7) |
60(5) |
10 |
20 |
20 (1) |
20 (1) |
18 |
20 |
20 (1) |
20 (3) |
32 |
20 |
18 (2) |
0 |
56 |
20 |
0 |
0 |
100 |
20 |
3 |
0 |
( ) = sticks to the surface but swimming
Description of key information
EC50 (48 h): 24.1 mg/L (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 24.1 mg/L
Additional information
The acute toxicity of the substance to aquatic invertebrates was investigated according to EU Method C.2 and GLP. Daphnia magna were exposed to nominal test substance concentrations of 10, 18, 32, 58 and 100 mg/L. The mobility of the Daphnids was determined after 24 and 48 hours.Analytical dose verification was not performed since the substance was expected to be stable under the tested conditions.
At test termination an EC50(48 h) of 24.1 mg/L based on nominal test substance concentration was determined (NOEC (48 h): 18 mg/L, nominal).Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.