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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
29-Feb-1984 to 01-Mar-1984
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only a summary report was available for review.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
1984

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Evaluation for cytopathic effect by placing the test material, octamethyltrisiloxane, in direct contact for 24 hours with a confluent monolayer of human embryonic cells in vitro.
GLP compliance:
yes
Remarks:
FDA GLP Regulations
Type of method:
in vitro
Endpoint addressed:
other: tissue cell biocompatibility

Test material

Constituent 1
Chemical structure
Reference substance name:
Octamethyltrisiloxane
EC Number:
203-497-4
EC Name:
Octamethyltrisiloxane
Cas Number:
107-51-7
Molecular formula:
C8H24O2Si3
IUPAC Name:
2,2,4,4,6,6-hexamethyl-3,5-dioxa-2,4,6-trisilaheptane

Test animals

Species:
human
Strain:
other: Flow 7000 human embryonic foreskin cells
Sex:
male

Administration / exposure

Route of administration:
other: in vitro
Vehicle:
unchanged (no vehicle)
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
24 hours
Frequency of treatment:
once
Post exposure period:
not applicable
Doses / concentrations
Remarks:
Doses / Concentrations:
no data
Basis:
no data
No. of animals per sex per dose:
not applicable
Control animals:
yes
other: positive control (unspecified)

Results and discussion

Details on results:
No cytopathic effect was observed after treatment with the test material [no details available].

Applicant's summary and conclusion

Conclusions:
Octamethyltrisiloxane was evaluated for its cytopathic effect in human embryonic cells. After 24 hours in direct contact, no cyotpathic effect was observed.
Executive summary:

Octamethyltrisiloxane (Dow Corning 200 Fluid, 1 CS) was tested for cytopathic effects in vitro in a non-guideline study conducted to GLP. It was applied in direct contact with a monolayer of cultured human embryonic cells and the cultures incubated at 37 °C for 24 hours. On microscopic examination there was no evidence of cytopathic effect in comparison with positive and negative controls.