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EC number: 203-497-4 | CAS number: 107-51-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted according to an appropriate OECD test guideline, and in compliance with GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was collected from the Cambridge Wastewater Treatment Facility, Cambridge, Maryland on March 30, 2009. The Cambridge facility treats predominantly residential wastes.
- Preparation of inoculum for exposure: The sludge was sieved using a 2-mm screen and then aerated at test temperature until its adjustment. The activated sludge was diluted in test medium to approximately 30 mg total suspended solids/L and aerated with CO2-free air until its adjustment. The inoculated medium used in the test was prepared by diluting the preconditioned activated sludge in test medium to 4 mg total suspended solids/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
TEST SYSTEM
- Culturing apparatus: glass serum bottles with a nominal volume of 160 mL. The chambers were sealed with butyl rubber septa and crimp caps.
SAMPLING
- Sampling frequency: Replicate chambers from the control, reference and treatment groups were removed for IC analysis once a week over the test period. Replicate chambers from the toxicity control group were removed for IC analysis on Days 1 and 28. Three replicate test chambers from each group were analysed at each respective sacrifice interval, except for the last interval (5 replicates each).
- Sampling method: Samples for analysis were prepared by converting CO2 to carbonate. One millilitre of 7M NaOH was injected into the test chambers, which were subsequently shaken for at least 1 hour at test temperature. After mixing, the chambers were removed from the shaker table, allowed to settle and then samples were removed for IC analysis.- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Test substance: -3.7% in 28 days
Reference substance: 96.5% in 28d (>60% in 7 days)
Toxicity control: 46.2% in 28d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Biodegradation of 0% in 28 days was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.
Reference
Table 1: Inorganic carbon concentration (mg C/l) and calculated % biodegradation for inoculum blank, reference substance, test substance and toxicity control
Type of suspension |
1d |
7d |
14d |
21d |
28d |
|
|||||
Inorg. C (mgC/l) |
(%) biodeg |
Inorg. C (mgC/l) |
(%) biodeg |
Inorg. C (mgC/l) |
(%) biodeg |
Inorg. C (mgC/l) |
(%) biodeg |
Inorg. C (mgC/l) |
(%) biodeg |
Cumulative average(%) biodeg(Day 28) |
|
|
|
|
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|
|
|
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Inoculum Blank |
- |
- |
0.3; 0.1; 0.4 |
- |
2.3; 2.4; 1.8 |
- |
2.9; 2.8; 3.1 |
- |
2.8; 2.8; 3.1; 2.6; 3.1 |
- |
- |
|
|
|
|
|
|
|
|
|
|||
Reference substance |
- |
- |
16.9; 16.8; 17.4 |
83.8; 83.4; 86.1 |
18.8; 19.5; 19.1 |
84.1; 87.4; 85.4 |
20.5; 20.4; 20.4 |
88.4; 88.2; 88.2 |
22.1; 22.0; 22.2; 21.9; 21.9 |
96.7; 96.3; 97.5; 96.1; 95.8 |
96.5 |
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|
|
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Test substance |
- |
- |
0.1; 0.0; 0.0 |
-0.7; -1.3; -1.5 |
1.9; 2.3; 1.5 |
-1.5; 0.4; -3.2 |
2.4; 2.3; 2.4 |
-2.9; -3.2; -2.6 |
2.2; 2.2; 2.4; 2.1; 2.0 |
-3.5; -3.7; -2.6; -3.9; -4.6 |
-3.7 |
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Toxicity control |
13.4; 13.6; 13.6 |
33.7; 34.3; 34.4 |
- |
- |
- |
- |
- |
- |
21.3; 21.1; 21.1; 21.4; 20.9 |
46.6; 46.0; 45.9; 46.8; 45.5 |
46.2 |
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Description of key information
Biodegradation in water: screening tests: 0% in 28 days (OECD 310)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Biodegradation of 0% in 28 days was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.
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