Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-864-0
CAS number: 75-35-4
* corrected values
This in vitro study was performed to assess
the corneal irritation and damage potential of 1,1-dichloroethylene by
means of the BCOP assay using fresh bovine corneae. After a first
opacity measurement of the fresh bovine corneae (t0), the neat test item
1,1-dichloroethylene, the positive, and the negative controls were
applied to corneae and incubated for 10 minutes at 32 ± 2 °C. The
posterior chamber contained MEM medium supplemented with sodium
bicarbonate and L-glutamine and 1% fetal calf serum (FCS) (complete
medium = cMEM). After the incubation phase the test item, the positive,
and the negative controls were each rinsed from the corneae and opacity
was measured again (t10). Further, the corneae were incubated for
another 120 minutes at 32 ± 2 °C in complete medium, and opacity was
measured a third time (t130). After the opacity measurements
permeability of the corneae was determined while application of 1 mL of
a fluorescein solution for 90 minutes at 32 ± 2 °C in a horizontal
position. The liquid coming out was measured spectrophotometrically.
With the negative control (0.9% NaCl solution) neither an increase of
opacity nor permeability of the corneae could be observed. The positive
control (2-Ethoxyethanol) showed clear opacity and distinctive
permeability of the corneae and therefore, is classified as severe eye
irritant. The test item 1,1-dichloroethylene caused only a slight
increase of opacity values but a distinct increase of the permeability
values of the corneae compared with the results of the negative control.
The calculated mean in vitro score was 43.90 and therefore, the test
item was classified as moderate eye irritant.
In conclusion, it can be stated that in this
study and under the experimental conditions reported, the test item
1,1-dichloroethylene is considered to be a moderate eye irritant.
Therefore the test item needs not be classified as GHS category 1, but
requires a classification as GHS category 2.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Šajā tīmekļa vietnē tiek izmantoti sīkfaili, lai nodrošinātu Jums vislabāko lietojumu mūsu tīmekļa vietnēs.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again