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Short-term toxicity to aquatic invertebrates

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In a key static 48 hourDaphnia magnaacute toxicity test (OECD 202, KS=1), five daphnids/ replicate were exposed to the Water Accommodated Fraction (WAF) of light cracked gas oil (MRD-10-595) at nominal concentrations of 0.05, 0.15, 0.45, 1.35 and 4.05 mg/L. The 48 hour EL50 was 0.32 mg/L based on mobility (ExxonMobil 2012a).

In a supporting static 48 hourDaphnia magnaacute toxicity test (OECD 202, KS=1), five daphnids/ replicate were exposed to the Water Accommodated Fraction (WAF) of light cracked gas oil (MRD-10-578) at nominal concentrations of 0.05, 0.15, 0.45, 1.35 and 4.05 mg/L. The 48 hour EL50 was 0.7 mg/L based on mobility (ExxonMobil 2012b).

In a supporting static 48 hourDaphnia magnaacute toxicity test (OECD 202, KS=1), five daphnids/ replicate were exposed to the Water Accommodated Fraction (WAF) of light catalytic cracked gas oil (MRD-10-576) at nominal concentrations of 0.102, 0.256, 0.640, 1.60 and 4.00 mg/L. The 48 hour EL50 was 0.51 mg/L based on mobility (ExxonMobil 2011c).

In a supporting static 48 hourDaphnia magnaacute toxicity test (OECD 202, KS=1), five daphnids/ replicate were exposed to the Water Accommodated Fraction (WAF) of light thermal cracked gas oil (MRD-10-604) at nominal concentrations of 0.32, 1.6, 8.0, 40 and 200 mg/L. The 48 hour EL50 was 5.8 mg/L based on mobility (ExxonMobil 2012c).

The aquatic toxicity was estimated using the PETROTOX computer model, which combines a partitioning model (used to calculate the aqueous concentration of hydrocarbon components as a function of substance loading) with the Target Lipid Model (used to calculate acute and chronic toxicity of non-polar narcotic chemicals). PETROTOX computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a petroleum substance and membrane-water partition coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism. The estimated freshwater invertebrate 48-hr EL50 value is 0.319 mg/L.