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Toxicity to aquatic algae and cyanobacteria

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Description of key information

No effects on aquatic algae up to the limit of the water solubility of Fatty Acid Glycerides were observed (NOEC ≥ water solubility).

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Additional information

Four studies with the freshwater algae Scenedesmus subspicatus are available for four different substances (CAS 73398-61-5, CAS 67701-30-8, CAS 65381-09-1 and CAS 620-67-7). In all four static studies with an exposition time of 72 hours no effect on Scenedesmus subspicatus was observed up to the limit of the water solubility of the tested substances.

Three tests were conducted according to EU-Method C.3 and one according to guideline 88/302/EWG, which is equivalent to OECD guideline 201. Two of the tests (CAS 67701-30-8 and CAS 65381-09-1) were conducted with additional consideration of the VCI concept for poorly soluble substances. Thus, two different test solutions were prepared with 100 mg/L: One solution where the test substance was directly weighed into the test vessel and undissolved substance was not removed and a second solution with separation of undissolved substance through filtration (preparation of a Water Accommodated Fraction (WAF)). No effect on the growth rate of Scenedesmus subspicatus was observed in the solutions prepared as WAF and thus, it can be stated that no effect up to the limit of the water solubility of the test substances occurred under conditions tested. The effects observed in the oversaturated solutions with undissolved test substance can therefore be attributed to physical impacts of the undissolved test substances and not to a systemic toxicity of the tested substances. The other two tests (CAS 73398 -61 -5 and CAS 620 -67 -7) used filtered test solutions (WAF) and did not observe effects on Scenedesmus subspicatus up to the limit of the water solubility of the substances either.

Thus, no effect up to the limit of the water solubility for all category members Fatty Acid Glycerides is assumed.