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Short-term toxicity to aquatic invertebrates

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Description of key information

No effect on aquatic invertebrates up to the limit of the water solubility of Fatty Acid Glycerides was observed (EC50 > water solubility).

Key value for chemical safety assessment

Additional information

Four short-term studies with Daphnia magna are available for four different substances (CAS 97722-02-6, CAS 67701-30-8, CAS 620-67-7 and CAS 65381-09-1). In all four static studies over 48 hours no effect on Daphnia magna was observed up to the limit of the water solubility of the tested substances.

All four tests were conducted according to EU-Method C.2, which is equivalent to OECD guideline 202. Two of the tests (CAS 67701-30-8 and CAS 65381-09-1) were conducted with additional consideration of the VCI concept for poorly soluble substances. Thus, two different test solutions were prepared: One solution where the test substance was directly weighed into the test vessel and undissolved substance was not removed and a second solution with separation of undissolved substance through filtration (preparation of a Water Accommodated Fraction (WAF)). No effect was observed up to the limit of the water solubility of the tested substances under conditions tested. Furthermore less than 30% of the Daphnids were immobilised in the oversaturated solutions with undissolved test substance. The other two tests (CAS 97722-02-6 and CAS 620-67-7) used filtered test solutions and did not observe effects on Daphnia magna either.

No effect up to the limit of the water solubility for all category members Fatty Acid Glycerides is assumed.