Humic acids, potassium salts

Short-term toxicity to aquatic invertebrates

Description of key information

EU Method C.2. - Acute toxicity for Daphnia, Directive 92/69/EEC, published in O.J. L 383A, 1992.
GLP study

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

116 mg/L

Additional information

The test substance was tested in acute immobilisation test on daphnia.

The preliminary test was performed in range of test substance nominal concentrations 1 – 100 mg·L^-1. The analytical results showed, that the composition of the dilution water has influence on the solubility of the test substance and the part of the test substance is precipitated at preparation of the stock solution. For this reason concentrations measured at the beginning of the test are lower than nominal concentration. Based on no toxicity of the test substance found in preliminary test, the limit test was performed.

The limit test was performed in range of nominal concentrations 61 – 150 mg·L^-1. The test was performed as static.

With regards to analytical results obtained by preliminary test, the analytical determination of the test substance concentrations was performed in each test concentrations at the beginning and at the end of the test.

For all evaluations and results were used the concentrations determined at the beginning of the test.

Test results:

24–hour EC₅₀ > 116 mg·L^-1 (determined concentration)

48–hour EC₅₀ > 116mg·L^-1 (determined concentration)

24–hour NOEC = 116 mg·L^-1 (determined concentration)

48–hour NOEC = 116 mg·L^-1 (determined concentration)

24–hour EC₁₀₀ > 116 mg·L^-1 (determined concentration)

48–hour EC₁₀₀ > 116 mg·L^-1 (determined concentration)