2-hydroxyethyl methacrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part 1 "Daphnia sp., Acute Immobilisation Test

GLP compliance:

yes

Specific details on test material used for the study:

Supplier: Wako Pure Chemical Ind.
Purity: 97.2 %

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The material of a necessary amount is dissolved to the dilution water, and examination field liquid 10,000 mg/L is adjusted. 

Test organisms (species):

Daphnia magna

Details on test organisms:

-Test Organisms: 
a) Age at Study Initiation: < 24 hours after hatching 
b) Supplier/Source: Supplied from U.S. EPA Environmental Research  Laboratory

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

55.6 mg/L

Test temperature:

20.0 - 20.2 degree C 

pH:

6.7 - 7.2

Dissolved oxygen:

8.4-8.7

Salinity:

Not Applicable

Nominal and measured concentrations:

95.3, 171, 309, 556, 1000 mg/L- nominal
97.0, 177, 318, 568, 1010 mg/L-TWM measured

Details on test conditions:

-Test Conditions: 
a) Dilution Water Source: Dechlorination water 
b) Dilution Water Chemistry: hardness= 55.6 mg/L CaCO3, pH= 7.7,  chlorine concentration < 0.02 mg/L 
c) Exposure Vessel Type: Petri dish (diameter = 8.5 cm, depth = 5.7 cm) 
d) Nominal concentrations (as mg/L): 95.3, 171, 309, 556, 1000 
e) Stock Solutions Preparation and Stability: The material of a  necessary amount is dissolved to the dilution water, and examination field liquid 10,000 mg/L is adjusted. 
f) Number of Replicates: 4 
g) Individuals per Replicate: 5 
h) Water Temerature Range: 20.0 - 20.2 degree C 
i) Light Conditions: 16:8 hours; light darkness cycle

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

171 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

380 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Binomial

Table 1: Measured concentration

Nominal        Measured conc. (mg/L) (Percentage of nominal)
conc.      --------------------------------------------------
(mg/L)         0 hour     48 hour      Time-weighted mean
-------------------------------------------------------------
Control         n.d.       n.d.            n.d.
-------------------------------------------------------------
  95.3         99.5(104)   94.4(99.1)      97.0(102)
-------------------------------------------------------------
  171          178(104)    175(102)        177(103)
-------------------------------------------------------------
  309          319(103)    317(103)        318(103)
-------------------------------------------------------------
  556          577(104)    558(100)        568(102)  
-------------------------------------------------------------
 1000          1010(101)  1010(101)       1010(101)
-------------------------------------------------------------
                                            n.d.: < 5.00 mg/L


Table 2: Water chemistry in test

Nominal           Measured value
conc.      --------------------------------------------------
(mg/L)         DO (mg/L)                   pH
           --------------------------------------------------       
            0 hour     48 hour          0 hour     48 hour  
-------------------------------------------------------------
Control     8.7        8.4              8.0        7.6
-------------------------------------------------------------
 95.3       8.7        8.4              7.2        7.2
-------------------------------------------------------------
 171        8.6        8.4              6.9        7.1
-------------------------------------------------------------
 309        8.6        8.4              6.8        7.1
-------------------------------------------------------------
 556        8.6        8.4              6.7        7.1        -------------------------------------------------------------
1000        8.6        8.5              6.7        7.0  
-------------------------------------------------------------


Table 3: Number immobility as compared to the  number exposed

Nominal        Cumulative number of immobilized
               Daphnia (Percentage immobility)
conc.      --------------------------------------------------
(mg/L)            24 hour               48 hour      
-------------------------------------------------------------
Control            0(0)                 0(0)
-------------------------------------------------------------
 95.3              0(0)                 0(0)
-------------------------------------------------------------
 171               0(0)                 0(0)
-------------------------------------------------------------
 309               0(0)                 3(15)
-------------------------------------------------------------
 556               8(40)                20(100)
-------------------------------------------------------------
1000               20(100)              20(100)
-------------------------------------------------------------

Validity criteria fulfilled:

yes

Conclusions:

The 48-hr EC50 was determined to be 380 mg/L in Daphnia magna.

Executive summary:

A 48-hour toxicity study with 2-hydroxyethyl methacrylate to daphnids (Daphnia magna) was conducted acc. OECD 202 (part 1) under static conditions with 4 replicates of 5 daphnids per test concentration exposed to a nominal test concentrations of 0 (control), 95.3, 171, 309, 556, and 1000 mg/l. Water samples were measured for analytical verification of test concentrations at 0 and 48 hours. The resulting time-weighted mean measured concentrations were 0 (control), 97.0, 177, 318, 568, and 1010 mg/l. Immobility was observed for 15, 100, and 100% of the daphnids at the 318, 568, and 1010 mg/L test levels, respectively. No daphnid immobility was observed in the control or remaining test levels during the expsosure. 

The 48-hour EC50 value was determined to be >380 mg/L.

Description of key information

A short-term toxicity test with Daphnia magna exposed to 2-hydroxyethyl methacrylate under static conditions, and following standard test guidelines (OECD 202, part 1), resulted in 48-hour EC50 value of 380 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Remarks:

48 h

Effect concentration:

380 mg/L