1,1,1-trifluoroethane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP report with certificate conducted at highly qualified laboratory.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1-trifluoroethane

EC Number:

206-996-5

EC Name:

1,1,1-trifluoroethane

Cas Number:

420-46-2

Molecular formula:

C2H3F3

IUPAC Name:

1,1,1-trifluoroethane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampled prior to study and at study termination (two times). Flasks were sealed during the study to prevent loss of test material.

Test solutions

Vehicle:

no

Details on test solutions:

Flasks were sealed during the study to prevent loss of test material.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Groups of 10 Daphnid less than 24-hrs old were tested in separate flasks. A total of 8 exposure levels (including a control) were evaluated in duplicate.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

Total alkalinity 84; EDTA hardness 77; Conductivity 160

Test temperature:

19.9 to 20.0 deg C

pH:

7.2 to 7.6

Dissolved oxygen:

reported to be below 40% saturation at 390 mg/l but with in acceptable range for all other exposure levels. at 390 mg/l 2.6 to 4.9 mg/l; at 62 mg/l 7.5 to 7.8 mg/l; at 13 mg/l 8.3 to 8.4 mg/l control 8.4 to 8.5 mg/l

Salinity:

data not available

Nominal and measured concentrations:

Nominal concentration(mg/l) 0(control), 16, 27, 45, 76, 144, 210, 350 analytical concentration(mg/l) 0(control) 13, 23, 31, 62, 140, 210, 390

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

other: 80% immobility

Effect conc.:

390 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The 48 -hr EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l under static conditions at 20 deg. C.

Executive summary:

EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l.under static conditions at 20 deg. C. The mean measured concentrations in the test flasks ranged from 81 to 111% of the nominal concentrations.