Triphenyl phosphite

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Triphenyl phosphite

EC Number:

202-908-4

EC Name:

Triphenyl phosphite

Cas Number:

101-02-0

Molecular formula:

C18H15O3P

IUPAC Name:

triphenyl phosphite

Details on test material:

- Name of test material (as cited in study report): Triphenyl phosphite
- Commercial purity: > 97% (Phosphorus content = 10.0 %)
- Lot/batch No.: TPPx-Z18-04080A
- Supplier: Borg Warner Company, Parkersburg, WV

Test animals

Species:

rat

Strain:

other: Sherman-Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 200 and 300 grams
- Housing: The animals were housed and maintained in compliance with the Animal Welfare Act (Pub. L-94-279) 9 CFR, Part 3.
- Fasting period before study: Animals were fasted overnight, but were not deprived of water.
- Diet: Following administration the animals were allowed food ad libitum
- Water: Following administration the animals were allowed water ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The sample was dosed as supplied (described as a colorless to straw-colored liquid).

Doses:

1.00, 1.26, 1.41, 1.58, 2.00, 2.51, 3.16, and 3.98 g/kg (males)
1.00, 1.26, 1.58, 1.78, 2.00, 2.51, 3.16, and 3.98 g/kg (females)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 21 days
- Frequency of observations and weighing: rats were observed daily for signs of toxicity and mortality.

Statistics:

The LD50 was calculated using the method described by Finney, D.J. 1971. ‘Statistical Methods in Biological Assay', 2nd Edition, London Griffen Press.

Results and discussion

Preliminary study:

In the initial limit dose experiment with 5 g/kg, within 1 hour the animals were depressed and ruffled, after 2-3 hours the animals were comatose. All animals died within 18 hours. Gross pathological examination revealed no remarkable findings. Because the LD50 was < 5 g/kg, a definitive LD50 study was performed.

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

1.59 other: g/kg

95% CL:

1.45 - 2.13

Mortality:

At the highest four doses in both males and females (2.00 to 3.98 g/kg) the animals died within 1-18 hours.

Clinical signs:

other: At the highest four doses in both males and females (2.00 to 3.98 g/kg), signs of toxicity (severe depression, ruffled appearance, drooling, ataxia) appeared within 1-2 hours, the animals became comatose within 2-3 hours. At the lower doses in males (1.26

Gross pathology:

Gross pathological examination revealed nothing remarkable.

Any other information on results incl. tables

The doses and number animals at each dose that died are presented below:

	Males				Females
Dose	Study days			Dose	Study days
	1	2	3 - 21		1	2	3 -21
1.00	0	0	0	1.00	0	0	0
1.26	0	0	0	1.26	1	0	0
1.41	1	0	0	1.41	1	0	0
1.58	2	0	-	1.58	3	0	0
2.00	5	-	-	2.00	5	-	-
2.51	5	-	-	2.51	5	-	-
3.16	5	-	-	3.16	5	-	-
3.98	5	-	-	3.98	5	-	-

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The LD50 (rat) of 1.59 g/kg bw, results in TPP being classified as a Category 4 Acute Toxicity based on teh GHS/CLP criteria.

Executive summary:

LD50 (rat) -1.59 g/kg bw - Category 4 Acute Toxicity.