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Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference
Reference Type:
publication
Title:
Differences in a2u-globulins increased in male rat kidneys following treatment with several a2u-globulin accumulating agents: cystein protease(s) play(s) an important role in production of kidney-type-a2u-globulin
Author:
Saito K, Kaneko H, Isobe N, Nakatsuka I, Yoshitake A and Yamada H
Year:
1992
Bibliographic source:
Toxicology 076, 177-186

Materials and methods

Type of study / information:
Nephrotoxicity
Principles of method if other than guideline:
The effects of alpha 2u-globulin accumulating agents on alpha2µ-globulins in rat kidneys were examined by SDS-PAGE and 
immunoblotting  analysis.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
purity > 97 % from Wako Pure Chemical Industries Ltd.

Results and discussion

Any other information on results incl. tables

Treatment of 3 male Sprague-Dawley rat with isophorone (150 mg/kg) by  gavage for 14 consecutive days resulted in a significant 

intensification  of a protein band corresponding to kidney-type-alpha2µ-globulin with a molecular mass calculated to be ~ 16kDa.

However, intraperitoneal treatment with leupeptin and E-64, well known cystein protease inhibitors, only slighthly increasing this kidney-type-alpha2µ-globulin band.

Applicant's summary and conclusion

Conclusions:
Isophorone form protein complexes with alpha2µ-globulin in vivo (SDS-page and immunoblotting after 14 days isophorone administration).
Executive summary:

Effects of alpha2µ-globulin accumulating agents on alpha2µ-globulins in rat kidneys were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting analysis. The treatment of male animals with isophorone (150 mg/kg) by gavage for 14 consecutive daysresulted in a marked intensification of a protein band corresponding to kidney-type-alpha2µ-globulin, with a molecular mass calculated to be ~ 16 kDa.