Bismuth vanadium tetraoxide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Bismuth vanadium tetraoxide

EC Number:

237-898-0

EC Name:

Bismuth vanadium tetraoxide

Cas Number:

14059-33-7

Molecular formula:

Bi O4 V

IUPAC Name:

vanadium(5+) bismuth(3+) tetraoxidandiide

Details on test material:

- Name of test material (as cited in study report): Bismuth Vanadate
- Molecular formula (if other than submission substance): BiVO4
- Molecular weight (if other than submission substance): 323.92
- Physical state: yellow powder
- Analytical purity: >99.5% w/w
- Impurities (identity and concentrations): water and others, <0.5% w/w
- Lot/batch No.: BVB-1018
- Stability under test conditions: stable
- Other: melting point>800°C

Method

Target gene:

bacterial reverse mutation assay

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

S9-mix from treated SD rats

Test concentrations with justification for top dose:

50, 100, 200, 500, 1000, 2000 and 5000 µg/plate in the first experiment (dose selection) and 313, 625, 1250, 2500 and 5000 µg/plate in the econdt experiment

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: solubility

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2AA for all strains with S9-mix; AF-2for TA100, WP2 uvrA and TA98, NaN3 for TA1535 and ICR-191 for TA1537 without S9-mix

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 min
- Incubation: 48 hours

DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency

Evaluation criteria:

not reported

Statistics:

not reported

Results and discussion

Test results

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and E. coli WP2 uvrA

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks:

Because there was no increase in the colony number of reverse mutation for every strain, comparing with negative control, we concluded that the mutagenicity of this test substance is negative (see Table 1&2)

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Remarks:

the test substance was tested up to 5000 µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

- Solubility of the test substance was less than 5 w/v % in water and DMSO and less than 10 w/v % in acetone (measured at this test institute)
- We selected DMSO as the solvent, because it made more stable suspension than water and acetone.
- We observed yellow precipitation of the test substance above 625 µg/plate without S9-mix and 313 µg/plate with S9-mix. But there was no obstacle to count the colony number of reverse mutation.

Any other information on results incl. tables

Table 1; first experiment: Dose selection result

 

With(+) or without (-) S9-mix		Test substance concentration (mg/plate	Mean number of revertants (number of colonies/plate; triplicate)
			Based-pair substitution type			Frameshift type
			TA100	TA1535	WP2 uvrA	TA98	TA1537
		Solvent control	102	9	24	25	10
		50	120	11	32	19	10
		100	113	10	22	20	12
		200	120	10	23	23	13
		500	112	14	22	20	10
		1000	103	10	23	20	8
		2000	109	10	19	20	11
		5000	99	11	19	22	10
		Solvent control	109	12	29	32	22
		50	115	8	35	43	18
		100	107	13	35	33	14
		200	102	10	36	31	20
		500	109	12	26	37	15
		1000	117	10	29	32	21
		2000	101	13	30	25	18
		5000	114	13	28	40	18
Pos. Ctrl	Without S9-mix	Name	AF-2	NaN3	AF-2	AF-2	ICR-191
		Concentration (µg/plate	0.01	0.5	0.01	0.1	0.1
		Mean number of colonies/plate	446	283	203	507	2178
	With S9-mix	Name	2AA	2AA	2AA	2AA	2AA
		Concentration (µg/plate	1	2	10	0.5	2
		Mean number of colonies/plate	520	160	580	163	135

Table 2; second experiment: Dose selection result

 

With(+) or without (-) S9-mix		Test substance concentration (mg/plate	Mean number of revertants (number of colonies/plate; triplicate)
			Based-pair substitution type			Frameshift type
			TA100	TA1535	WP2 uvrA	TA98	TA1537
S9-mix (-)		Solvent control	114	10	27	24	14
		313	117	12	32	22	13
		625	123	9	23	24	15
		1250	134	11	22	27	13
		2500	116	10	27	20	13
		5000	129	9	22	24	14
S9-mix (+)		Solvent control	111	10	30	30	21
		313	126	11	38	37	26
		625	133	10	38	35	28
		1250	128	11	39	36	27
		2500	127	9	33	37	20
		5000	117	10	31	34	25
Pos. Ctrl	Without S9-mix	Name	AF-2	NaN3	AF-2	AF-2	ICR-191
		Concentration (µg/plate	0.01	0.5	0.01	0.1	0.1
		Mean number of colonies/plate	515	282	532	2572	2178
	With S9-mix	Name	2AA	2AA	2AA	2AA	2AA
		Concentration (µg/plate	1	2	10	0.5	2
		Mean number of colonies/plate	545	129	601	190	130