Registration Dossier
Registration Dossier
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EC number: 204-464-7 | CAS number: 121-32-4
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Inhibitory effects of flavourings on mutagenesis induced by chemicals in bacteria.
- Author:
- Ohta T, Watanabe M, Watanabe K, Shirasu Y and Kada T
- Year:
- 1 986
- Bibliographic source:
- Food and Chemical Toxicology, 24(1), 51-54
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Two bacterial strains were used: E. Coli (uvrA, trpE) and salmonella thyphimurium TA98 (uvrB, hisD).
In brief, bacterial cultures were washed twice and treated with one of the mutagens in phosphate buffer for 15-60 min, after which the cells were washed again to remove the mutagen and then assayed for numbers of mutants and viable cells. Mutagen-treated cells were spread onto semi-enriched
minimal (SEM) agar plates containing a flavouring at an appropriate concentration or onto control plates containing no flavouring.
For E. Coli, the SEM agar medium consisted of Vogel-Bonner E medium supplemented with 0.4% glucose, 1.5% agar and 5% (v/v) liquid nutrient broth, while the supplement for S. Thyphimurium were 0.4% glucose, 1.5% agar and 50 µM biotin.
After appropriate dilution of the bacterial suspension, viable cells were determined on the SEM plates. Plates were incubated at 37°C for 3 days, and
then colonies of trp+ or his+ revertants and viable cells were counted. - GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- 3-ethoxy-4-hydroxybenzaldehyde
- EC Number:
- 204-464-7
- EC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Cas Number:
- 121-32-4
- Molecular formula:
- C9H10O3
- IUPAC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Details on test material:
- no data
Constituent 1
Results and discussion
- Details on results:
- Ethylvanillin showed a clear antimutagenic effect on 4-NQO-induced mutagenesis in E. Coli.
A pronounced decrease in the number of trp+ revertant colonies was observed on the plates containing ethylvanillin (50-150 µg/ml).
There was no effect with S. thyphimurium TA98.
Applicant's summary and conclusion
- Conclusions:
- antimutagenic effects of ethyl vanillin on 4-NQO-induced mutagenesis in E. Coli.
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