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EC number: 237-864-5
CAS number: 14025-15-1
The study by Geerts (2010) is considered key due to the GLP guideline study wrt active sludge respiration inhibition, 3h duration. The NOEC was detemined >= 640 mg/L for EDTA-MnNa2. This would correspond to >= 654 mg/L EDTA-CuNa2.
To investigate toxicity to microorganisms
from different sources, multiple endpoints have been investigated with
several EDTA salts or complexes. Respiration rate, bioluminescense,
nitrification inhibition and growth of Pseudomonas have been
investigated, but not all studies are equally reliable.
Geerts (2010) is preferred, supported by Hu
(2003), BASF (1988) & van Ginkel (2000) all indicating that effects of
the EDTA salts or complexes are not harmful to activated sludge.
To determine a PNEC for microorg the study
by Hu (nitrification inhibition) should also be considered as this
endpoint is generally considered more sensitive than sludge respiration.
The EC50 endpoint was determined at 2.4 mmol/L for EDTA-H4 (701 mg/L)
corresponding to 953 mg/L EDTA-CuNa2.
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