2-Propanol, 1,1'-(tridecylimino)bis-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Part 15

Principles of method if other than guideline:

The stability of the test substance in test water without fish was investigated in a separate study for the time period of 96 hours (Project No.: 99F0460/905174, 10 Feb 1995) on request of the "Bundesanstalt fuer Arbeitsschutz, Anmeldestelle Chemikaliengesetz (BAU)".

GLP compliance:

no

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

N-Tridecyldiisopropanolamine
Batch No.: 87/70046
Test Substance No.: 88/222
Purity: > 96 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

The compound was dissolved in acetone (1.0 g/100 ml) and added to the test water. Analogously to the highest amount of solvent used, 1.0 ml acetone/L of test water was tested as solvent control. No undissolved test substance was visible.

Test organisms

Test organisms (species):

Leuciscus idus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Nominal and measured concentrations:

Based on the results of 2 range finding studies, the test substance concentrations were fixed as follows: 0, 0.215, 0.464, 1.00, 2.15, 4.64, and 10 mg/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

4.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal Dead fish after
conc. 1h 4h 24h 48h 72h 96h
[mg/l]
-----------------------------------------------------------
0.215 0/10 0/10 0/10 0/10 0/10 0/10
0.464 0/10 0/10 0/10 0/10 0/10 0/10
1.000 0/10 2/10 3/10 3/10 3/10 3/10
2.150 0/10 2/10 5/10 7/10 7/10 7/10
4.640 0/10 10/10 10/10 10/10 10/10 10/10
10.000 10/10 10/10 10/10 10/10 10/10 10/10

control 0/10 0/10 0/10 0/10 0/10 0/10
control* 0/10 0/10 0/10 0/10 0/10 0/10
-----------------------------------------------------------
* = solvent control

Intoxication symptoms:
----------------------
Tumbling, narcotic-like state, convulsions