Benzoic acid

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Limited study summary, no guideline and no GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Study predates approved guidelines.Five male and 5 female rats of the Spartan strain was used in this test. The group of 10 rats was placed in a sealed 59.1 liter glass chamber and exposed for 4 hours to a dynamic atmosphere containing the dust of 12200 mg/m3 test substance.

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzoic acid

EC Number:

200-618-2

EC Name:

Benzoic acid

Cas Number:

65-85-0

Molecular formula:

C7H6O2

IUPAC Name:

benzoic acid

Details on test material:

Batch No.: 09-I2-28Purity: not specified

Test animals

Species:

rat

Strain:

other: Spartan

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Weight at study initiation: from 226 to 244 grams- Fasting period before study: not indicated- Housing: Animals were housed by sex in groups of 5 in metal cages above the dropping.- Diet (e.g. ad libitum): Purina Laboratory Chow- Water (e.g. ad libitum): ad libitumENVIRONMENTAL CONDITIONS- Temperature (°C): controlled- Humidity (%): controlled- Air changes (per hr): No data- Photoperiod (hrs dark / hrs light): no data

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

whole body

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION- Exposure apparatus: Wright Dust Feeder- Exposure chamber volume: 59.1 liter- Method of holding animals in test chamber: closed chamber with seperated 4 units of 2 or 3 rats each- Source and rate of air: Dried and filtered air was passed through the mechanism and directly into the exposure chamber.- Method of conditioning air: Airflow was regulated by means of a flowmeter.- System of generating particulates/aerosols: Wright Dust Feeder- Method of particle size determination: not performed

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

approximately 12200 mg/m3 (The physical properties of the test material preclude the administration of the test compound at higher atmospheric concentrations.)

No. of animals per sex per dose:

5 male and 5 female per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days - Frequency of observations and weighing: 4 hr, 24 hr and 14 days- Necropsy of survivors performed: yes- Other examinations performed: clinical signs, body weight

Statistics:

None stated

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 12 200 mg/m³ air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

There were no deaths following a single inhalation dose of the test substance at 12200 mg/m3 air.

Clinical signs:

other: Increased motor activity and slight erythema were observed during the 4 hour exposure period. After 4 hours exposure, 1 rat exhibited salivation. After 24 hours and after 14 days all animals appeared normal.

Body weight:

Normal body weight gain was observed.

Gross pathology:

No information provided.

Other findings:

No information provided.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated informationCriteria used for interpretation of results: EU

Conclusions:

The LC50 was considered to be >12,200 mg/m3 air. The test substance would not be considered a highly toxic material by the inhalation route of administration.

Executive summary:

Five male and five female rats of the Spartan strain was used in this test. The group of 10 rats was placed in a sealed 59.1 liter glass chamber and exposed for 4 hours to a dynamic atmosphere containing the dust of 12200 mg/m3 test substance.

The LC50 was considered to be >12,200 mg/m3 air. The test substance would not be considered a highly toxic material by the inhalation route of administration.