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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: 2b. Guideline Study with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
yes
Remarks:
(see below)
Principles of method if other than guideline:
Slight modifications according to Van Leeuwen, C.J., J.L. Maas-Diepeveen, G. Niebeek, W.H.A.Vergouw, P.S. Griffioen and M.W. Luyken, 1985. Aquatic toxicological aspects of dithiocarbamates and related compounds. Short-term tests. Aquat. Toxicol. 7, 145-164.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
Aniline: purity = pro analysis; LogPoct=1.03 (0.90); Mol weight= 93.13;Chs= 0.00
2-methylaniline: purity >= 99.5%; LogPoct=1.54; Mol weight= 107.16;Chs= 0.10
3-methylaniline: purity >= 99%; LogPoct=1.54; Mol weight= 107.16;Chs= - 0.07
4-methylaniline: purity > 97%; LogPoct=1.54; Mol weight= 107.16;Chs= -0.17
Chs: Hammett sigma constant, which is dependent on the position of the substituent.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): isopropanol

Test organisms

Test organisms (species):
Chlorella pyrenoidosa

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
94 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: Aniline; 95% C.I.:77-114 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
55 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 2-methylaniline; 95% C.I.: 40-259 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 3-methylaniline: 95% CL: 40-48 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
138 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 4-methylaniline: 95% CL: 130-148 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
All anilines tested were slightly toxic to green algae with EC50 values for growth between 10 and 100 mg/L , except for 4-methylaniline with an EC50 > 100 mg/L.