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Environmental fate & pathways

Hydrolysis

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Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: 2e. Study well documented, meets generally accepted principles of measurement of biodegradation using a method different from those recommended by international guidelines.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
Use of an automated headspace method.
Principles of method if other than guideline:
A chemical control was tested simultaneously in a biodegradation test with activated sludge to determine degradation in the absence of inoculum. In fact this control evaluates the hydrolytical stability of the test substance under the test conditions applied.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Reagent-grade (Kodak Laboratory Chemicals)
Radiolabelling:
no

Study design

Analytical monitoring:
yes
Details on sampling:
Samples had been acidified by injection of 0.5 ml 2 N phosphoric acid and filtered through 0.22-pm membrane filters prior to analysis.
Buffers:
Not applicable.
Estimation method (if used):
Not applicable.
Duration of test
Duration:
21 d
Initial conc. measured:
20 mg/L
Number of replicates:
3 for each time point.
Positive controls:
no
Negative controls:
no
Statistical methods:
Not applied.

Results and discussion

Preliminary study:
Not applicable.
Test performance:
The results refer to abiotic degradation, which may include hydrolysis, but determaination of hydrolysis was not the ultimate endpoint of the experiment.
Transformation products:
no
Total recovery of test substance (in %)
% Recovery:
ca. 92
pH:
7
Temp.:
25 °C
Duration:
21 d
Dissipation DT50 of parent compound
Key result
pH:
7
Temp.:
25 °C
Remarks on result:
other: N-methylaniline was not degraded under the test conditions

Applicant's summary and conclusion

Conclusions:
N-methylaniline showed no significant abiotic degradation in water during 21 days of exposure at pH of 7.0 ± 0.2 and a temperature of 25 ± 2 °C.