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EC number: 203-227-5
CAS number: 104-68-7
One guideline study on freshwater green algae
The purpose of this study was to assess the potential effects of DiEPh
on the growth of the freshwater green alga, Pseudokirchneriella
subcapitata. Algal growth was evaluated over a 96 -hour (4 day) exposure
period at nominal concentrations of 0 (medium control), 31.3, 62.5, 125,
250, 500, and 1000 mg DiEPh/L. The medium control level included six
replicate vessels and the treatment concentrations included three
replicate vessels per level, all inoculated at an intial nominal loading
rate of 10,000 cells/ml per vessel. In addition, blank vessels were set
at each level (including the control) to serve as counting blanks to
correct the daily counts for potential interference of the isoton
solution and/or test material, as well as to monitor pH and
concentration of the test material without the algal biomass. Cell
density determinations were made at approximately 24, 48, 72 and 96
hours of exposure.
Analytical verification of the test solution concentrations was
performed on samples collected on days 0 (test initiation) and 4 (test
termination) of the study, Measured DiEPh concentrations ranged from
95.5 to 98% of nominal on day 0 and, in replicates with algae, ranged
from 91.7 to 100% of nominal on day 4. The resulting mean measured
concentrations were < LLQ (medium control), 29.7, 59.7, 121, 247, 496
and 984 mg DiEPh/L
Temperatures during the exposure period ranged from 23.8 to 24 C. Light
intensity for inoculated replicates ranged from 4900 lux to 5000 lux.
The pH value ranged from 7.5 to 7.6 at test initiation, from 7.7 to 8.5
in pooled replicates with algae on day 3, and from 7.7 to 8.3 in pooled
replicates with algae and 7.6 to 7.7 in blank replicates without algae
at test termination.
The results, based on mean measured concentrations of DiEPh, were as
- 72 -hour ErC50 (95% Confidence Interval) = 393 (364, 422) mg/L
- 96 -hour ErC50 (95% Confidence Interval) = 396 (355, 437) mg/L
- 72 -hour EyC50 (95% Confidence Interval) = 254 (249, 260) mg/L
- 96 -hour EyC50 (95% Confidence Interval) = 269 (266, 272) mg/L
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