trisodium 2-(2-sulfoethoxy)ethane-1-sulfonate ethenesulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov 2022

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

semi static , with renewal of the test solution after 48 hours
test concentration 100 mg/L, limit test
blank control

GLP compliance:

yes

Specific details on test material used for the study:

Reaction mass of disodium 2,2 oxydiethanesulfonate and sodium ethenesulfonate
Batch lot number:202206140027
Dry weight content : 33.94 %, correction factor is 2.94 The test item is a multi-constituent
Composition.
Constituent1:Sodium ethylene sulphonate cas 3039-83-6: 74.84 %
Constituent 2 : Isethionate bisether, disodium salt cas 63440-92-6: 15.18%
Impurity 1:Isethionate sodium cas 1562-00-1: 5.15%
Impurity 2:Sodium ethionate, disodium salt cas1562-03-4: 0.48 %
Impurity 3:Sodium sulfate cas 7757-82-6 :3.12 %
Impurity 4:Sodium ethandisulfonate disodium salt cas 5325-43-9:0.49 %

The test material has a purity of 33.94 % dose calculations were corrected for purity ( correction factor 2.946).

Analytical monitoring:

yes

Details on sampling:

Sampling for analysis:
Freshly prepared solutions (t=0 h and t=48 h) and 48-hour
old solutions (t=48 h and t=96 h)

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Carp (Cyprinus carpio, Teleostei Cyprinidae).
Source ‘De Haar Vissen’, Zodiac proefacc. Wageningen University & Research, The Netherlands
Mean length 2.4 ± 0.1 cm.
Mean weight 0.17 ± 0.04 g.
Characteristics F1 from a single parent-pair bred in UV-treated water.
Reason for selection This system has been selected as an internationally accepted species. Healthy fish supplied with a health certificate.
Total fish used 14

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

total exposure duratio,nis 96 h, with renewal of test solutions after 48 hours

Post exposure observation period:

not applicable

Hardness:

180 mg/L expressed as CaCo3

Test temperature:

20-22n °C (table 5)

pH:

pH: 6.0-8.5, constant within 1 unit (table 3)

Dissolved oxygen:

oxygen > 60% of air saturation

Nominal and measured concentrations:

The results of analysis of the samples taken during the limit test are described in Table 3 and Table 4 of the appended Analytical Report.
Samples taken from the limit concentration and the control were analyzed for two monitored constituents in the test material. The measured concentrations in the freshly prepared solutions at the start of the test and at the 48-h renewal were generally around nominal at the limit concentration for both constituents. The 48-hour old solutions showed that the concentrations remained stable over time.
Based on these results, effect parameters were expressed as analytically confirmed nominal concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel:6.5 liters, all-glass vessels
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 5.0 liters, glass
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): semi static
- Renewal rate of test solution (frequency/flow rate): renewal after 48 h
- No. of organisms per vessel: 7/ teqst group
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):no verhicle used
- Biomass loading rate:0.24 g fish/liter, i.e. 7 fish per 5.0 liters of test medium.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
The following salts were added to the test medium.
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L



- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement: Conductivity, pH, nitrate, nitrite, and ammonia concentration: once a week. Temperature was continuously recorded. In addition, pH and temperature were measured before transferring the fish to the test system.

OTHER TEST CONDITIONS
- Adjustment of pH: not applicable
- Photoperiod:
- Light intensity: Due to the light sensitivity of the test material, the test was performed under dimmed light conditions (32-45 lux).

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: a correction factor was used for the dry content of the substance .(2.94)
- Justification for using less concentrations than requested by guideline:
- Range finding study ; see results in appendix 2
- Test concentrations: limit concentration 100 mg/l
- Results used to determine the conditions for the definitive study: see appendix 2 ,results of DRF

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

no mortality was observed up to 100 mg/L therefore the LC50 was established as >100 mg/l

Details on results:

- Other abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Hatching rate control and treatment groups (embryo):
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium:no test substance is highly soluble in water.

Results with reference substance (positive control):

not applicable

Validity criteria fulfilled:

yes

Remarks:

1. No mortality was observed in the control throughout the test. 2. The dissolved oxygen concentration has been at least 60% of the air saturation value throughout the test (>5 mg/L at 22°C). 3. Test concentrations have been analytically measured.

Conclusions:

In conclusion, the 96h-LC50 for carp (Cyprinus carpio) exposed to Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate ( EC 700-978-5) was beyond the limit concentration tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.

Executive summary:

The objective of the study was to evaluate the test material for its ability to generate acute toxic effects in Carp (Cyprinus carpio) during an exposure period of 96 hours and, if possible, to determine the LC₅₀ at all observation times.

The design of this study is in compliance with the following study guidelines:

OECD Guideline 203. "Fish, Acute Toxicity Test", 2019.

The batch of Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate tested was a clear light-yellow liquid with dry residue percentage of 33.94% dissolved in water and was visually completely soluble in test medium at the concentration tested. A correction was made for the water content of the test material. A correction factor of 2.946 was used. All concentrations reported are based on dry residue.

Preparation of test solutions started with the limit concentration of 100 mg/L applying an approximately 30-minute period of magnetic stirring to accelerate dissolution of the test material in medium. The limit concentration was clear and colorless at the end of the preparation procedure.

The results of analysis of the samples taken during the limit test are described in Table 3 and Table 4 of the analytical report.

The  test duration was 96 hours, and the test type is semi-static, with  renewal of the  test solution after 48 hours.

Samples taken from the limit concentration and the control were analyzed for two monitored constituents in the test material. The measured concentrations in the freshly prepared solutions at the start of the test and at the 48-h renewal were generally around nominal at the limit concentration for both constituents. The 48-hour old solutions showed that the concentrations remained stable over time.

Based on these results, effect parameters were expressed as analytically confirmed nominal concentration

Mortality and Sublethal effects.

Table 1 shows the mortality data and sublethal effects recorded during the limit test. 

No mortality or sublethal effects were observed in the control and at the limit concentration throughout the exposure period.

The responses recorded in this test were in agreement with what was expected based on the results of the non-GLP preliminary range finding test (Test Facility Refence No. 20332246; Appendix 2

Determination of the effect concentrations.

Table 2 shows the effect parameters based on analytically confirmed nominal concentrations.

Experimental conditions

The results of measurement of pH and dissolved oxygen concentrations are presented in Table 3 and Table 4, respectively.

The temperature continuously measured in a temperature control vessel varied between 20 and 22°C. The temperatures measured during the study in the various test vessels are presented in Table 5. All test conditions remained within the ranges prescribed by the study plan (pH: 6.0-8.5, constant within 1 unit; temperature 20-24°C, constant within 2°C; oxygen > 60% of air saturation).All validity parameters were met.

In conclusion, the 96h-LC₅₀ for carp (Cyprinus carpio) exposed to Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate ( EC 700-978-5) was beyond the limit concentration tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.

 

Description of key information

In conclusion, the 96h-LC50 for carp (Cyprinus carpio) exposed to Reaction mass of disodium 2,2’-oxydiethanesulfonate and sodium ethenesulfonate ( EC 700-978-5) was beyond the limit concentration tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L