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EC number: 277-874-7 | CAS number: 74441-06-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-11-08 to 2009-11-12
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-amino-N-[4-(aminocarbonyl)phenyl]benzamide
- EC Number:
- 277-874-7
- EC Name:
- 4-amino-N-[4-(aminocarbonyl)phenyl]benzamide
- Cas Number:
- 74441-06-8
- Molecular formula:
- C14H13N3O2
- IUPAC Name:
- 4-amino-N-(4-carbamoylphenyl)benzamide
- Details on test material:
- - Name of test material (as cited in study report): p-Aminobenzoylaminobenzamid TF
- Molecular formula: C14H13N3O2
- Molecular weight: 255.28 g/mol
- Physical state: Solid, beige powder
- Analytical purity: 96.44 % (w/w)
- Purity test date: 2009-04-29
- Expiration date of the lot/batch: 2011-04-29
- Storage condition of test material: Room temperature, protected from moisture and light
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The saturated solution and the control were analytically verified via HPLC after 0 hours (fresh media) and after 48 hours (old media).
- Sampling method: For the old media, separate replicates without test organisms were prepared and incubated under test conditions. The recovery rates of the active ingredient should be within ± 20 % of the initially measured value.
- Sample storage conditions before analysis:All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the twenty fold of the water solubility (3.1 mg/L) of the test item was prepared with dilution water two days prior to application. Therefore 62 mg/L of the test item was weighed out.
- Eluate: Dilution water
- Differential loading: The saturated solution* was tested as limit concentration
- Controls: Dilution water without test item tested under the same conditions as the test groups.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water at test start: 263 (mg CaCO3/L)
- Test temperature:
- 20.5 °C
- pH:
- Water Quality Parameters at Test Start (0 hours)
(measured in one additional replicate of the saturated solution and the control)
pH-Value
Saturated Solution 7.53
Control 7.98
Water Quality Parameters at the Test End (48 hours)
(measured in all replicates)
pH-Values
Replicates
1 2 3 4
Saturated Solution 7.52 7.58 7.57 7.56
Control 7.47 7.51 7.51 7.53 - Dissolved oxygen:
- Water Quality Parameters at Test Start (0 hours)
(measured in one additional replicate of the saturated solution and the control)
Dissolved
O2-Concentration
[mg/L]
Saturated Solution 7.27
Control 8.37
Water Quality Parameters at the Test End (48 hours)
(measured in all replicates)
Dissolved O2 –Concentration [mg/L]
Replicates
1 2 3 4
Saturated Solution 7.54 7.50 7.42 7.57
Control 7.32 7.27 7.49 7.47 - Salinity:
- Freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Acc. to Directive 92/69/EEC Method C.2, Annex 1
- Conductivity: Dilution water at test start: 640 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daphnid immobility was determined in all groups after 24 hours and 48 hours. An animal was considered to be immobile, if it was not able to swim within 15 seconds after gentle agitation of the test vessel.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:2
- Range finding study
Immobilization Rates of the Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Dilution level
of the saturated solution IMMOBILIZATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
1:1 0 0 0 0 0 0
1.10 0 10 5 0 10 5
1.100 0 0 0 0 0 0
Control 0 0 0 0 0 0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.22 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: 0%
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.67 (CI 1.63 - 1.71) mg/L - Reported statistics and error estimates:
- The effects of the saturated solution were evaluated directly from the observation data.
EC50-value was only calculated for the reference item by sigmoidal dose-response regression. The confidence interval for EC50 were calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism.
Any other information on results incl. tables
Percentage of Daphnids Incapable of Swimming after 24 and 48 h of Exposure
(n = 20, divided into 4 replicates with 5 daphnids each)
IMMOBILIZATION [%] |
||||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
Saturated solution |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution* of the test item p-Aminobenzoylaminobenzamid TF, corresponding to the initially measured concentration of 1.22 mg/L, no effect was observed.
- Executive summary:
In the acute immobilization test to Daphnia magna (STRAUS) the effects of the saturated solution* of the test item p-Aminobenzoylaminobenzamid TF with a nominal loading of 62 mg/L, was determined according to OECD 202 (2004). The limit test was conducted under static conditions over a period of 48 hours .
20 test organisms were exposed to the saturated solution* and the control. The saturated solution* and the control were analysed via HPLC after 0 hours (fresh media) and 48 hours (old media). The final recovery rate of the test item in the saturated solution* was 98 % of the initially measured concentration. All effect values given are based on the initially measured test item concentration. Water quality parameters (pH-value and dissolved oxygen concentration), measured in the fresh (0 hours) an old media (48 hours), were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
EC-Values, NOEC and LOEC
(based on the initially measured test item concentration)
Test Duration
[h]Initially Measured
Test Item Concentration[mg/L]
Confidence Interval
p = 95%
[mg/L]EC50
24
> 1.22
not applicable
48
> 1.22
not applicable
EC100
24
> 1.22
---
48
> 1.22
---
NOEC
48
1.22
---
LOEC
48
> 1.22
---
corresponding to the initially measured concentration of 1.22 mg/L,
no effect was observed.
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