Poly[oxy(methyl-1,2-ethanediyl)], .alpha.-hydro-.omega.-[2-[[2,2-dimethyl-3-[(1-oxododecyl)oxy]propylidene]amino]methylethoxy]-, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

The acute toxicity of the test substance to Daphnia magna was tested under semi-static conditions according to OECD guideline 202. The EC50 (48 h) was calculated to be 27 mg/L. The NOEC was calculated to be 4.6 mg/L.

 

Toxicity to aquatic algae and cyanobacteria

The purpose of this study was to determine the effect of the test item SIKA Hardener LTJ (SIKAHärterLTJ) on the growth of a unicellular green algal species Raphidocelis subcapitataaccording to OECD guideline 201. The calculated biological endpoints are EC10 (growth rate) 0.58 mg/L, EC50 (growth rate) 1.70 mg/L, EC10 (yield) 0.27 mg/L, EC50 (yield) 0.70 mg/L, NOEC (growth rate) 0.4 mg/L and NOEC (yield) 0.1 mg/L.

Additional information

Short-term toxicity to aquatic invertebrates

The acute toxicity of the Water Accommodated Fraction (WAF) of Sika Hardener LTJ to Daphnia magna (water flea) was tested under semi-static conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2. The test item is immiscible with water and therefore a WAF was prepared. Due to the hydrolysis of SIKA Hardener LTJ no analytical method for the determination of the concentration was available. Thus, the nominal concentrations were used for calculation. The EC50 (48 h) was calculated to be 27 mg/L. The highest concentration causing no immobility (no observed effect concentration, NOEC) after 48 hours amounted to 4.6 mg/L. The EC100 was 100 mg/L, which was observed after 48 hours. All validation criteria were fullfilled.

 

Toxicity to aquatic algae and cyanobacteria

The purpose of this study was to determine the effect of the test item SIKA Hardener LTJ (SIKA Härter LTJ) on the growth of a unicellular green algal species Raphidocelis subcapitata (formerly known as Pseudokirchneriella subcapitata). Exponentially growing cultures of Raphidocelis subcapitata were exposed to various concentrations of the test item over several generations under defined conditions. The algal growth in relation to a control culture was determined over a fixed test period of 72 hours and thus, over several algal generations. The test method of application and the test species Raphidocelis subcapitata are recommended by the test guidelines. Based on the results of non-GLP Preliminary Range-Finding Test the following six test concentrations in a geometric series (with a spacing factor of 4) were tested: 0.1, 0.4, 1.6, 6.3, 25, 100 mg/L (loading rate). The test concentrations were prepared using the WAF method (by preparing individual WAFs). Due to the rapid hydrolysis of the test item (SIKA Hardener LTJ (SIKA Härter LTJ)) validation of suitable analytical method was not possible for the verification of test item concentration in aqueous test media (Study number 644-100-0646). Analytical determination of the test item concentrations was not feasible, therefore biological endpoints of the test are based on the nominal test item concentrations (loading rate). Biological results are based on the nominal test item concentrations (loading rate).For the determination of the LOEC and NOEC, the calculated mean growth rates and yield at the test concentrations were tested on significant differences to the control values by Bonferroni t-Test using TOXSTAT software. The test item had a statistically significant inhibitory effect on the growth of Raphidocelis subcapitata after the exposure period of 72 hours based on the average specific growth rate in concentrations of 1.6, 6.3, 25 and 100 mg/L and based on yield in concentrations of 0.4, 1.6, 6.3, 25 and 100 mg/L (Bonferroni t-Test, 1 tailed, α=0.05) when compared to control. The ErCx and EyCx values of the test item and their confidence limits were calculated using Probit analysis by TOXSTAT software. The calculated biological endpoints are EC10 (growth rate) 0.58 mg/L, EC50 (growth rate) 1.70 mg/L, EC10 (yield) 0.27 mg/L, EC50 (yield) 0.70 mg/L, NOEC (growth rate) 0.4 mg/L and NOEC (yield) 0.1 mg/L.