Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: limited documentation, short report, pre- GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- no
- Remarks:
- pre-dates GLP
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- pale yellow scales
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
- Details on test animals or tissues and environmental conditions:
- Food and water ad libitum
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 100 mg per eye
- Duration of treatment / exposure:
- 24 h
- Observation period (in vivo):
- 72 h
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- treated eyes were rinsed with 0.9 5 saline after 24 h, Observtions were done at 1, 7, 24 ,48, and 72 hours.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 74
- Reversibility:
- not specified
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 52
- Reversibility:
- not specified
- Remarks on result:
- other: Own scale. Estimated score according to report details
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 10
- Reversibility:
- not specified
- Remarks on result:
- other: Own scale. Estimated score according to report details
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 14
- Reversibility:
- not specified
- Remarks on result:
- other: Own scale. Estimated score according to report details
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Remarks on result:
- not measured/tested
Applicant's summary and conclusion
- Interpretation of results:
- highly irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- 1-Chloro-2,4-dinitrobenzene was highly irritating in the rabiit eye after 24 h tretment using the pure material.
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