3-methylbutanone

Short-term toxicity to aquatic invertebrates

Description of key information

A pre-GLP, static, limit study and a modern OECD 202 static renewal study conducted according to GLP. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Two aquatic invertebrate studies are available for Methyl Isopropyl Ketone (MIPK). The first study was a pre-GLP study conducted as a limit test at a nominal 100 mg/L concentration under static, non-renewal conditions with simultaneous exposure of 6 invertebrate species and one fish species. In this study daphnia were exposed for 48 hours and all of the other species were exposed for 96 hours. 50% mortality did not occur in any of the species tested, therefore the LC50 was reported as >100 mg/L. Due to concerns for volatility of the test substance, a recent OECD 202 study with daphnia was conducted using a static renewal system with multiple concentrations up to 100 mg/L nominal. In this study initial and final test solutions were measured daily. Some losses of the test substance occurred during the study and the mean measured concentration for the highest exposure was 81 mg/L. 5% immobilization was observed in the highest concentration but this was not considered an adverse response from exposure to the test substance. Therefore, the NOEC was reported as 81 mg/L and the LC50 was > 81 mg/L, the highest concentration tested. Based upon a weight of evidence from these two studies it is believed that the LC50 for aquatic invertebrates is ≥ 100 mg/L.