1-cyanoallyl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

adopted May 26, 1983; not tested in TA 102 or E.coli WP2 uvrA

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-cyanoallyl acetate

EC Number:

239-743-2

EC Name:

1-cyanoallyl acetate

Cas Number:

15667-63-7

Molecular formula:

C6H7NO2

IUPAC Name:

1-cyanoprop-2-en-1-yl acetate

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

s9 liver microsomal fraction from rat

Test concentrations with justification for top dose:

Exp. 1 plate incorporation: 3.3 to 100 ug/plate,
Exp. 2: pre-incubation test: 6.6-333 ug/plate (TA 1535, TA1537), 1.0-100 (TA98, TA100)

Vehicle / solvent:

water

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

water

Positive controls:

yes

Remarks:

positive controls as seen below

Controls 2

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

without S9 mix : TA 1535, TA100

Controls 3

Positive controls:

yes

Positive control substance:

other: 4-Nitro-o-phenylene-diamine

Remarks:

TA1537, TA98, without S9mix

Controls 4

Positive controls:

yes

Positive control substance:

other: 2-Aminoanthracene

Remarks:

with S9 mix

Details on test system and experimental conditions:

METHOD OF APPLICATION: Experiment 1: in agar (plate incorporation); Experiment 2: preincubation


DETERMINATION OF CYTOTOXICITY
- Method: reduction in the number of revertants

Evaluation criteria:

- negative control in the laboratory historical range for each tester strain
- positive control chemicals produces responses in all tester strains within laboratory historical range
- Test substance is considered as mutagenic if:
- a dose-related and reproducible increase in the number of revertants occurs
- a substantial and reproducible increase for at least one test concentration (TA98, TA100 twofold increase, TA1535, TA1537 threefold increase compared to the corresponding solvent control)
Mean plate count should be at least three times the concurrent vehicle control group mean
- selected dose range should include a clearly toxic concentration or should exhibit limited solubility as demonstrated by the preliminary toxicity range-finding test or should extend to 5 mg/plate

Statistics:

none

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

in concentrations above 100 ug/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Applicant's summary and conclusion

Conclusions:

Interpretation of results:
negative

Under the conditions tested, the test substance did not cause gene mutations in the genome of the tester strains used.