Potassium cyanate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2009-03-09 to 2009-06-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

adopted 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Version / remarks:

2008/440/EC

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Defined amounts of test item were directly weighed into the designated test flasks to reach the planned nominal concentrations. The nominal test item concentrations were prepared by mechanical dispersion (using orbital shaker). These test solutions were freshly prepared at the beginning of the experiment, in the testing laboratory.

Test organisms (species):

activated sludge, domestic

Details on inoculum:

The activated sludge was supplied from the sewage plant for domestic sewage in Veszprém, Hungary.
The activated sludge used for this study was washed and centrifuged and the liquid phase was decanted. The solid material was re-suspended in isotonic saline solution and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and a ratio of wet sludge to its dry weight determined. Based on this ratio, calculated amounts of wet sludge were suspended in isotonic saline solution to yield a concentration equivalent to 4 g per litre (on dry weight basis). The pH of the activated sludge inoculum was determined to be pH 7.41. The activated sludge was used directly after conditioning.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

no post exposure period

Hardness:

no data

Test temperature:

19.8 – 20.3 °C (during the incubation) and
18.8 – 20.6 °C (during oxygen measurement)

pH:

The pH of the activated sludge inoculum was determined to be pH 7.41.

Dissolved oxygen:

The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 6.6 mg O2/L.

Salinity:

no data

Nominal and measured concentrations:

Before the start of the test defined amounts of the test item were directly weighed into the test flasks and mechanically dispersed using orbital shaker.
The test concentrations (10, 31, 100, 313 and 1000 mg/L) were chosen to permit the determination of the EC50. Concentrations in excess of nominal 1000 mg test item/L were not tested.
Two controls (deionised water, synthetic sewage and inoculum, but without addition of the test item) were tested in parallel.

Details on test conditions:

One test solution with a final volume of 330 mL was tested per treatment in a glass flask. 10.56 mL synthetic sewage and an adequate amount of the test item or an adequate volume of the stock solution of the reference item was filled up with deionised water to 198 mL before the start of the test. At the start of the test 132 mL activated sludge inoculum with a sludge concentration of 4 g/L (dry weight) was added, first to first control (C1), then in time intervals of 15 minutes (an arbitrary but convenient interval) to the test solutions of the reference item and the test item and finally to a second control (C2).
For the measurement of the respiration rate a well-mixed sample of each treatment was poured into a BOD flask after exactly 3 hours incubation time, and was not further aerated. The oxygen concentration was measured with a stirring O2 electrode and was recorded for about ten minutes. The oxygen consumption (in mg O2 L-1 minute-1) was determined from the most linear part of the respiration curve.
The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The temperature was measured in the climate chamber with a min/max thermometer during the incubation period. The water temperature was recorded during the oxygen measurement in all BOD bottles.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited in the concentration of range of 10-31 mg potassium cyanate /L. Slight stimulation of respiration rate was observed (-1.8 %) at the concentrations of 10 mg/L. The respiration rate was inhibited between 1.8 % and 17.5 % in the examined nominal test concentration range. Concentrations exceeding 1000 mg/L nominal were not tested.
Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50 and EC80 were higher than 1000 mg/L.
The NOEC was determined to be 1000 mg/L.
The NOEC for respiration rates was not based on the results of a statistical analysis, but it is biologically justified. The respiration rates were not inhibited and influenced dose dependently in the whole concentration range, and the observed slight inhibition (17.5 %) at the highest examined concentration level of 1000 mg/L was evaluated as reflecting the biological variability in the test. The calculated respiration rate, 0.470 (at 1000 mg/L) was in the historical control data range (0.514 ± 0.077), and the deviation from the control was slightly higher than 15 %, but still it can be considered as a biological variability of the test system.

Results with reference substance (positive control):

The 3-hour EC50 of the reference item 3,5-Dichlorophenol for the used activated sludge batch was determined to be 13.58 mg/L and thus the EC50 was in the acceptable the range of 5 to 30 mg/L.

Reported statistics and error estimates:

Standard deviation, EC50 calculatioins

Validity criteria fulfilled:

yes

Conclusions:

Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50 and EC80 were higher than 1000 mg/L and a NOEC of 1000 mg/L can be derived from this study..

Executive summary:

In a 3-h activated sludge test, an inoculum (domestic) were exposed to potassium cyanate at nominal concentrations of 10, 31, 100, 313 and 1000 mg/L under static conditions. 

In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited in the concentration of range of 10 - 31 mg potassium cyanate/L. Slight stimulation of respiration rate was observed (-1.8 %) at the concentrations of 10 mg/L. The respiration rate was inhibited between 1.8 % and 17.5 % in the examined nominal test concentration range. However, the respiration rates cease well within the historical control range for test concentrations. Concentrations exceeding 1000 mg/L nominal were not tested.

Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50and EC80 were higher than 1000 mg/L.

The NOEC was determined to be 1000 mg/L.

Description of key information

Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50 and EC80 were higher than 1000 mg/L. The NOEC was determined to be 1000 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

Key study:

In a 3-h activated sludge test, an inoculum (domestic sewage treatment plant) was exposed to potassium cyanate at nominal concentrations of 10, 31, 100, 313 and 1000 mg/L under static conditions. 

In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited in the concentration of range of 10 - 31 mg potassium cyanate/L. Slight stimulation of respiration rate was observed (-1.8 %) at the concentrations of 10 mg/L. The respiration rate was inhibited between 1.8 % and 17.5 % in the examined nominal test concentration range. Concentrations exceeding 1000 mg/L nominal were not tested.

Based on measured inhibition rates it can be stated that the 3-hour EC20, EC50and EC80 were higher than 1000 mg/L.

The NOEC was determined to be 1000 mg/L.