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EC number: 814-155-4 | CAS number: 13188-82-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 OCTOBER 2018 - 10 OCTOBER 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- OECD No. 209 Commission Regulation (EC) No 2016/266, C.11
- Deviations:
- yes
- Remarks:
- The start value of oxygen content for measurement of oxygen consumption was lower than 7 mg/L. TS3: 6.6 mg/L. An influence to the results of this study can be excluded.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- - Activated sludge suspension from the aeration tank of the wastewater treatment plant of Mannheim, Germany was sieved in the test facility. The suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water and the suspension was adjusted to 3 g/L Dw.
An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.
Test Concentrations: 1000, 500, 250, 125, 62.5 mg/L as nominal concentration
based on test substance without correction of purity and blank controls.
100, 10, 1 mg/L as nominal concentration based on reference substance (positive control).
Test replicates: 6 replicates for the control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration. - Vehicle:
- no
- Details on test solutions:
- 1000.4 mg of the reference substance was added to about 800 mL of deionized water and stirred at room temperature until the reference substance was completely dissolved.
The pHvalue of the stock solution was measured and adjusted to 7.4 with 1 M sodium hydroxide solution.
Following this the stock solution was made up to 1L with deionized water. The stock solution appeared yellowish and no undissolved reference substance was visible. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Reason for the selection of the test system: required by the test guidelines
Age: 1 day
Origin: aeration tank of the wastewater treatment plant of Mannheim, Germany
Collection of the test system: 09 Oct 2018
Arrival in the test facility: 09 Oct 2018
After arrival of the activated sludge suspension in the test facility the suspension was sieved
with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over
night at room temperature. At the next day the sludge suspension was washed once with
drinking water and the suspension was adjusted to 3 g/L Dw. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18.9-19.6 °C
- pH:
- 7.3 - 8.3
- Nominal and measured concentrations:
- 1000, 500, 250, 125, 62.5 mg/L as nominal concentration
based on test substance without correction of purity and blank
controls.
100, 10, 1 mg/L as nominal concentration based on reference
substance. - Details on test conditions:
- Incubation time: 3 hours
Test temperature: 18.9 – 19.6 °C
Test vessels: Glas-beakers (nominal volume 1L)
Test volume: 500 mL
Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD medium
Oxygen concentration during aeration: > 2 mg/L
Oxygen concentration immediately before measurement: > 7 mg/L with the exceptions described in remark test guideline deviations
Duration of the measurement of oxygen consumption: About 8 - 10 min
Sludge concentration in the test vessel: 1.5 g/L Dw - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 720 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Confidence Limits (95%): Lower: 579.9, upper: 955.8
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: Confidence Limits (95%): Lower: 92.1, upper 268.4
- Details on results:
- Please see Table 4.
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was 5.1 mg/L.
- Results with reference substance valid? Yes - Reported statistics and error estimates:
- The consumption rates were used for the determination of the ECx by the probit method based on Finney [1971] with the software TOXRAT Professional 2.10 [2001-2010]. The effect concentrations were given with an accuracy of 2 significant digits.
- Validity criteria fulfilled:
- yes
- Remarks:
- The coefficient of variation of the six replicates of blank control was 3.9 % O2 consumption. The EC50 of 3,5-dichlorophenol was 5.1 mg/L The mean oxygen uptake of the blank controls was 22 mg/g*h The test conformed to all validity criteria and is valid.
- Conclusions:
- EC10 = 190 mg/L, EC20 = 300 mg/L, EC50 = 720 mg/L, EC80 = > 1000 mg/L were found in the activated sludge respiration inhibition test with 3 hour residence time for the test substance. All validity criteria were met.
Reference
Table 4: Effect concentrations of the test item.
Toxicity Response Variable | Based on nominal effect concentration [mg/L] | Confidence Limits (95%) lower/upper |
EC10 | 190 | 92.1/268.4 |
EC20 | 300 | 186.3/385.7 |
EC50 | 720 | 579.3/955.8 |
EC80 | > 1000 | ---- |
The value of effect concentrations of EC10 EC20 EC50 were given with an accuracy of 2 significant digits. The degree of inhibition was evaluated by Probit analysis according to Finney.
Description of key information
In a 3-hr acute toxicity study sewage sludge according to OECD 209,
microorganisms were exposed to the test item at nominal concentrations
of 0, 62.5, 125, 250, 500 and 1000 mg/L.
The EC 50 value based on respiration rate after was 720 mg/L
(95 % confidence limits 579.3 - 955.8 mg/L) after a 3 h contact time.
The EC 10 was determined as 190 mg/L (95 % confidence limits 92.1 - 268.4 mg/L).
This toxicity study is classified as acceptable and satisfies the guideline requirements for OECD 209 toxicity
study. All validity criteria were met.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 720 mg/L
- EC10 or NOEC for microorganisms:
- 190 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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