Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 939-714-0 | CAS number: 1474044-77-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start 12 February 2013 - Completion 22 April 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without deviations affecting integrity of the results.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Deviations:
- no
- Principles of method if other than guideline:
- The (modified) study procedures described in this report were modified and originally based on the Organization for Economic Co-operation and Development (OECD), OECD guidelines for Testing of Chemicals, Section 3, Degradation and Accumulation, guideline No. 310: "Ready Biodegradability: CO2 in sealed vessels (Headspace Test)" adopted March 23, 2006.
In addition, the modified procedures were originally designed to meet the test methods prescribed by the following guideline:
• ISO Standard 14593 (1999) : Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test).
• OPPTS 835.3215 Inherent Biodegradability – Concawe test.
• Proposal for a new OECD guideline 302D – Inherent biodegradability – Concawe test (draft document October 2001).
The major modifications on the procedure were:
-Pre adaptation of sludge during a 13-day period, before the start of the test.
-Prolongation of the test period up to 56 days. - GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', Heeswijk-Dinther, The Netherlands, receiving predominantly domestic sewage.
- Duration of test (contact time):
- ca. 56 d
- Initial conc.:
- ca. 20 mg/L
- Based on:
- ThIC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Reference substance:
- other: 1-octanol
- Preliminary study:
- A 28-day biodegradation study was conducted prior to this test (see Biodegradation in water: screening tests.001)
- Test performance:
- The test proceded normally without significant deviations
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 0
- Sampling time:
- 56 d
- Results with reference substance:
- 1-octanol showed 72.9 degradation by exposure day 14.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Not inherently biodegradable under the conditions of the test.
- Executive summary:
An inherent biodegradation study was conducted with di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) in sealed vessels with headspace test analysis of C02. The study procedures were modified and originally based onthe OECD guideline No. 310, 2006. In addition, the modified procedures were originally designed to meet the test methods of theISO 14593 (1999), OPPTS 835.3215 and the proposal for a new OECD guideline 302D (2001). The major modifications on the procedure were the re adaptation of sludge during a 13-day period, before the start of the test and prolongation of the test period up to 56 days.
The batch of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) tested was a brown highly viscous liquid and a UVCB substance (treated as 100% pure). di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) as supplied contained 68% TOC.
Since, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was poorly soluble in water, 3.2 mg test substance,corresponding toa final organic carbon concentration of 20 mg C/l, was added directly to the test medium (final volume bottle 107 ml). The substance was incubated in a buffer-mineral salts medium inoculated with a mixed population of micro-organisms. Note that the test substance was added immediately before closing the bottles. The test was performed in sealed bottles with a headspace of air.
The test consisted of 3 groups:
1. Blank control: bottles containing inoculated medium;
2. Procedure control: bottles each containing inoculated medium and 1-Octanol at 20 mg C/l;
3. Test substance: bottles each containing inoculated medium and di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) at 20 mg C/l;
The CO2evolution resulting from the aerobic biodegradation of the test substance was determined by measuring the inorganic carbon (IC) produced in the test bottles in excess of that produced in blank vessels containing inoculated medium only. The extent of biodegradation was expressed as a percentage of the theoretical maximum IC production, based on the quantity of test substance (as organic carbon) added initially.
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA). Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) is designated as not inherently biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 6, 2012 to May 22, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP OECD guideline study without deficiencies that affected study validity
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- >= 19.5 mg/L
- Based on:
- ThIC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)),
- 1 positive control (sodium acetate) and
- 1 toxicity control (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) plus sodium acetate). - Preliminary study:
- No preliminary test was conducted.
- Test performance:
- 1. The positive control substance was biodegraded by at least 60% (73%) within 14 days.
2. The difference of duplicate values for %-degradation of the test substance was always less than 20.
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/l (46 mg CO2 per 2 litres of medium, corresponding to 23 mg CO2/l).
4. The Inorganic Carbon content (IC) of the test substance (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli-RO water (Millipore Corp., Bedford, Mass., USA, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from the test substance, 13 mg TOC/l).
Since all criteria for acceptability of the test were met, this study was considered to be valid. - Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 14 - <= 17
- Sampling time:
- 29 d
- Details on results:
- See results table
- Results with reference substance:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 14 and 17% biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA), for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
In the toxicity control more than 25% biodegradation occurred within 14 days (33%, based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under the conditions of the test, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not readily biodegradable.
- Executive summary:
A ‘ready’ biodegradability (modified Sturm test) was conducted with di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA). The study procedures described in this report were based on the OECD guideline No. 301 B, 1992. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Publication No.L142, Part C.4-C and the ISO International Standard 9439, 1999 and ISO Standard 10634, 1995.
di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was a brown highly viscous liquidwith a purity of provisional 84%. The test substance was tested in duplicate at 19.5 mg/l, corresponding to 13 mg TOC/l. The organic carbon content was based on the molecular formula and the purity of the testsubstance. The Theoretical CO2production (ThCO2) of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was calculated to be 2.49 mg CO2/mg.
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA)),
- 1 positive control (sodium acetate) and
- 1 toxicity control (di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) plus sodium acetate).
Since di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. Test duration was 28 days (last CO2-measurement on the 29thday).
The relative biodegradation values calculated from the measurements performed during the test period revealed 14 and 17% biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA), for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met. In the toxicity control, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was designated as not readily biodegradable.
Referenceopen allclose all
Percentages biodegradation
Nominal day |
% Biodegradation |
|
Reference substance |
di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) |
|
1 |
1.3 |
1.4 |
7 |
n.d. |
1.7 |
14 |
72.9 |
3.2 |
21 |
n.d. |
0 |
28 |
n.d. |
3.0 |
35 |
n.d. |
2.4 |
42 |
n.d. |
0 |
49 |
n.d. |
0.0 |
56 |
n.d. |
0 |
n.d.: not determined
Negative values are expressed as 0.
Table 1 CO2 production and percentage biodegradation of the test substance (bottle A). |
|
|||||||
|
Day |
HCl (0.05 N) titrated (ml) |
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
|
Blank (mean) |
bottle A |
||||||
|
2 |
47.89 |
47.09 |
0.79 |
0.9 |
0.9 |
1 |
|
|
5 |
47.04 |
45.71 |
1.33 |
1.5 |
2.3 |
3 |
|
|
7 |
47.15 |
45.91 |
1.24 |
1.4 |
3.7 |
4 |
|
|
9 |
46.78 |
45.83 |
0.95 |
1.0 |
4.7 |
5 |
|
|
14 |
46.06 |
45.29 |
0.77 |
0.8 |
5.6 |
6 |
|
|
19 |
44.81 |
43.71 |
1.10 |
1.2 |
6.8 |
8 |
|
|
23 |
44.98 |
43.41 |
1.57 |
1.7 |
8.5 |
10 |
|
|
27 |
45.32 |
44.23 |
0.00 |
0.0 |
8.5 |
10 |
|
|
29 |
45.93 |
44.93 |
1.00 |
1.1 |
9.6 |
11 |
|
|
29 |
48.63 |
47.12 |
1.51 |
1.7 |
11.3 |
13 |
|
|
29 |
49.53 |
48.46 |
1.07 |
1.2 |
12.5 |
14 |
|
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance: 87.3 mg CO2/2l |
|||||||
Table 2 CO2 production and percentage biodegradation of the test substance (bottle B). |
|
|||||||
|
Day |
HCl (0.05 N) titrated (ml) |
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
|
Blank (mean) |
bottle B |
||||||
|
2 |
47.89 |
47.49 |
0.39 |
0.4 |
0.4 |
0 |
|
|
5 |
47.04 |
46.21 |
0.82 |
0.9 |
1.3 |
2 |
|
|
7 |
47.15 |
45.75 |
1.40 |
1.5 |
2.9 |
3 |
|
|
9 |
46.78 |
46.10 |
0.68 |
0.7 |
3.6 |
4 |
|
|
14 |
46.06 |
44.78 |
1.28 |
1.4 |
5.0 |
6 |
|
|
19 |
44.81 |
43.15 |
1.66 |
1.8 |
6.9 |
8 |
|
|
23 |
44.98 |
43.35 |
1.63 |
1.8 |
8.6 |
10 |
|
|
27 |
45.32 |
43.11 |
2.21 |
2.4 |
11.1 |
13 |
|
|
29 |
45.93 |
44.60 |
1.33 |
1.5 |
12.5 |
14 |
|
|
29 |
48.63 |
46.86 |
1.77 |
1.9 |
14.5 |
16 |
|
|
29 |
49.53 |
48.76 |
0.77 |
0.8 |
15.3 |
17 |
|
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test substance:88.2 mg CO2/2l |
Description of key information
A ‘ready’ biodegradability (modified Sturm test) was conducted with di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA). Since di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. The relative biodegradation values calculated from the measurements performed during the test period revealed 14 and 17% biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA), for the duplicate bottles tested. In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was designated as not readily biodegradable.
An inherent biodegradation study was conducted withdi C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) in sealed vessels with headspace test analysis of C02. Since, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) was poorly soluble in water, 3.2 mg test substance,corresponding toa final organic carbon concentration of 20 mg C/l, was added directly to the test medium (final volume bottle 107 ml). The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA).
In conclusion, di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid (DNNSA) is designated as not inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.