Reaction mass of ethyl 2-ethyl-6,6-dimethylcyclohex-2-enecarboxylate and ethyl 2,3,6,6-tetramethylcyclohex-2-enecarboxylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 July to 07 August 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate

EC Number:

261-020-5

EC Name:

Ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate

Cas Number:

57934-97-1

Molecular formula:

C13H22O2

IUPAC Name:

ethyl 2-ethyl-6,6-dimethylcyclohex-2-ene-1-carboxylate

Constituent 2

Reference substance name:

Ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate

EC Number:

278-776-7

EC Name:

Ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate

Cas Number:

77851-07-1

Molecular formula:

C13H22O2

IUPAC Name:

ethyl 2,3,6,6-tetramethylcyclohex-2-ene-1-carboxylate

Specific details on test material used for the study:

Test material name (as stated in report): Givescone
Batch No. 261026
Appearance: Colourless to yellow pale liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva) was used. The sludge is collected in the morning , washed three times in the mineral medium (by centrifuging at 1000g for 10 minutes , discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

aniline

Remarks:

100 mg/l

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Givescone undergoes no biodegradation after 28 days in the test conditions. Thus, Givescone should be regarded as not readily biodegradable according to this test. The curves obtained with Aniline alone and with Givescone + Aniline show no significant toxic effect of Givescone on the micro-organisms at the test concentration.

Executive summary:

The ready biodegradability of Givescone has been determined by the Manometric Respirometry test according to the OECD Guideline No. 301 F.

Givescone undergoes no biodegradation after 28 days in the test conditions. Thus, Givescone should be regarded as not readily biodegradable according to this test. At the concentration used in the test (100 mg/l), Givescone is not inhibitory to the micro organisms.