2-Propenoic acid, (1-methyl-1,2-ethanediyl) bis[oxy(methyl-2,1-ethanediyl)] ester, reaction products with diethylamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 20, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

activated sludge

Details on inoculum:

- Source of inoculum: Activated sIudge from Iaboratory wastewater plants treating municipal sewage- Preparation of inoculum for exposure: Inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for about 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test temperature:

20 ± 2 °C

pH:

7.2-7.5

Dissolved oxygen:

> 6.5 mg/L

Nominal and measured concentrations:

1,000 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: - Type: Erlenmeyer-vessel (nominal volume 250 mL)- No. of vessels per control (replicates): Triplicate- No. of vessels per blank control (replicates): Triplicate

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Effective concentrations after 30 min incubation time:- EC20; EC50 and EC80 > 1,000 mg/L (nominal)

Results with reference substance (positive control):

Effective concentrations after 30 min incubation time:- EC20 = 3.3 mg/L; EC50 = 23 mg/L; EC80 = 82 mg/L (nominal)

Validity criteria fulfilled:

yes

Remarks:

Deviation of blank controls < 15 %; EC50 of 3,5-dichlorophenol was 5-30 mg/L.

Conclusions:

Under the test conditions, the EC20, EC50 and EC80 (30 min) values of the test substance were determined to be > 1,000 mg/L.

Executive summary:

A study was performed to assess the inhibitory effect of amine synergist on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209, EU Method C.11 and ISO 8192.

Activated sIudge from municipal sewage was exposed to the test substance (1,000 mg/L) for 30 min and analysed for oxygen consumption rate. 3,5-dichlorophenol was used as reference material. The EC50 (30 min) of the reference material was estimated to be within 5-30 mg/L.

Under the test conditions, the EC20, EC50 and EC80 (30 min) values of the test substance were determined to be > 1,000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

A study was performed to assess the inhibitory effect of amine synergist on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209, EU Method C.11 and ISO 8192. The EC20, EC50 and EC80 (30 min) values of the substance were > 1,000 mg/L (nominal) (Werner, 1999).