2-Propenoic acid, (1-methyl-1,2-ethanediyl) bis[oxy(methyl-2,1-ethanediyl)] ester, reaction products with diethylamine

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 October 1999 - 14 October 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION- Method: Test material was stirred in demineralized water for about 48 h to prepare a stock solution (nominal concentration = 125 mg/L)

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM- Source (laboratory, culture collection): Obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and was kept in liquid culture in the Laboratory of Ecology at BASF AG Ludwigshafen

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

21-25 °C

pH:

8.0-8.8

Nominal and measured concentrations:

Nominal concentrations: 6.25, 12.5, 25, 50 and 100 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel: - Material, size, headspace, fill volume: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable silicon sponge caps- Initial cells density: 10,000 cells/mL- No. of vessels per concentration (replicates): Triplicate- No. of vessels per control (replicates): Triplicate- No. of vessels per vehicle control (replicates): TriplicateGROWTH MEDIUM- Standard medium used: YesOTHER TEST CONDITIONS- Photoperiod: Artificial Iight, type universal white (e.g. OSRAM L 25), permanent illumination- Light intensity and quality: 60-120 µE/m2.s at a wavelength of 400-700 nmEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: After 72 hours in a counting chamber (Neubauer improved) in replicate 1 of the inoculated control.- Chlorophyll measurement: In vivo chlorophyll-a-fluorescence (pulsed excitation with Iight flashes having a wavelength of 435 nm); fluorescence measured after 0, 24, 48 and 72 hours.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

72 h

Dose descriptor:

other: EbC10

Effect conc.:

23.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

other: EbC50

Effect conc.:

41.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

other: EbC90

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Key result

Duration:

72 h

Dose descriptor:

other: ErC10

Effect conc.:

31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

other: ErC90

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

EC50 (72 h) = 0.35 mg/L

Reported statistics and error estimates:

Duncan multiple range test was carried out at a 95 % significance level.

Validity criteria fulfilled:

yes

Remarks:

EC50 (72 h) of the control substance potassium dichromate was 0.35 mg/L; cell multiplication factor in the untreated control was 180 fold after 72 h; variation of the pH-values during the test was not more than 2 units

Conclusions:

Under the test conditions, the 72 h EbC50 of the test substance to the algae Scenedesmus subspicatus was 41.6 mg/L (nominal). The 72 h ErC50 was > 100 mg/L (nominal). The 72 h NOEC and LOEC were estimated to be 12.5 or 25 mg/L (nominal), respectively.

Executive summary:

A study was performed to assess the acute toxicity of amine synergist to the green algae Scenedesmus subspicatus according to OECD Guideline 201, EU Method C.3 and EPA OPPTS 850.5400. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. Under the test conditions, the 72 h EbC50 of the test substance was 41.6 mg/L (nominal) based on biomass. The 72 h ErC50 was > 100 mg/L (nominal) based on growth rate. The 72 h EC10 was estimated to be 31 mg/L (nominal) based on growth rate (Reuschenbach, 1999).

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

EC10 or NOEC for freshwater algae:

31 mg/L

Additional information

A study was performed to assess the acute toxicity of amine synergist to the green algae Scenedesmus subspicatus according to OECD Guideline 201, EU Method C.3 and EPA OPPTS 850.5400. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. Under the test conditions, the 72 h EbC50 of the test substance was 41.6 mg/L (nominal) based on biomass. The 72 h ErC50 was > 100 mg/L (nominal) based on growth rate. The 72 h EC10 was estimated to be 31 mg/L (nominal) based on growth rate (Reuschenbach, 1999).