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EC number: 436-060-3 | CAS number: - FC 84508 PK
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Aug. 28, 2000 to Oct. 12, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- Commission Directive 88/302/EEC; Official Journal of the EC L 133 Part C (1988)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing
- Incubation time: 3 h with permanent aeration - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Method of cultivation: aeration tank of a waste water treatment plant treating predominantly domestic sewage (Wupper area water authority)
- Pretreatment: aeration ofthe activated sludge; daily feed with synthetic medium
- pH of the suspension before application: 6.8
- Test concentration of the activated sludge: 400 mg/L suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No data
- Test temperature:
- 20±2 °C
- pH:
- 7.4-7.7
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 1000, 1800, 3200, 5600, 10000 mg/L
- Details on test conditions:
- Type of application: Direct weighing
Test concentration of the activated sludge: 400 mg/L suspended solids - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (97 % purity)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Test substance showed 32.1 % respiration inhibition of activated sludge at a test substance concentration of 10000 mg/L
- Results with reference substance (positive control):
- - Results with reference substance valid: Yes
- Ref. concentrations: 2.5, 5, 10, 20, 40 mg/L
- % Inhibition: 86.2 and 92.2 at 20 and 40 mg/L concentrations.
- EC50: 7.9 mg/L - Reported statistics and error estimates:
- The EC values are determined by statistical methods (probit analysis).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the test substance showed 32.1 % respiration inhibition of activated sludge at a concentration of 10000 mg/L. The EC50 was determined to be > 10000 mg/L.
- Executive summary:
A study was performed to assess the toxicity of test substance to bacteria according to Commission Directive 88/3021EEC; Official Journal of the EC L133 Part C (1988) and equivalent to OECD guideline 209 in compliance with GLP
The activated sludge was exposed to the test substance at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 h. The test substance showed 32.1 % respiration inhibition on activated sludge at a concentration of 10000 mg/L. The EC50 could not be determined but would hypothetically be > 10000 mg/L.
Reference
Table 1. Results
Test concentration (mg/L) |
Respiratory rate Test substance (mg/L. h) |
Phys.-chem. O2-consumption (mg/L. h) |
Respiratory rate Phys.-chem. O2-consumption (mg/L. h) |
Inhibition (%) |
1000 |
42.0 |
0.0* |
42.0 |
0.0 |
1800 |
39.0 |
0.0* |
39.0 |
0.0 |
3200 |
38.4 |
0.0* |
38.4 |
0.0 |
5600 |
31.2 |
0.0* |
31.2 |
18.5 |
10000 |
26.0 |
0.0 |
26.0 |
32.1 |
Control, Mean |
38.3 |
|
||
Control 1 |
37.5 |
|
||
Control 2 |
39.0 |
|
* The physico chemical oxygen consumption is determined at 10000 mg/L test substance concentration. No physico-chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico chemical oxygen consumption (deduced values).
Description of key information
The EC50 of the test substance could not be determined. The hypothetical EC50 for respiration inhibition in microorganisms would be > 10000 mg/L
Key value for chemical safety assessment
Additional information
A study was performed to assess the toxicity of test substance to bacteria according to Commission Directive 88/3021EEC; Official Journal of the EC L133 Part C (1988) and equivalent to OECD guideline 209 in compliance with GLP
The activated sludge was exposed to the test substance at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 h. The test substance showed 32.1 % respiration inhibition on activated sludge at a concentration of 10000 mg/L. The EC50could not be determined but would hypothetically be > 10000 mg/L.
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