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EC number: 290-714-0 | CAS number: 90218-39-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts
- EC Number:
- 215-397-8
- EC Name:
- Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts
- Cas Number:
- 1325-54-8
- Molecular formula:
- Molecular formula of the main constituents: (1) C38H24N8Na4O12S4 (2) C52H32N10Na6O18S6
- IUPAC Name:
- Reaction mass of Tetrasodium 5-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]-2-[2-[2-sulfonato-4-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]phenyl]ethenyl]benzenesulfonate and Hexasodium 2-((E)-2-sulfonato-4-((E)-(3-sulfonato-4-((E)-2-sulfonato-4-((E)-(4-((E)-(4-sulfonatophenyl)diazenyl)phenyl)diazenyl)styryl)phenyl)diazenyl)styryl)-5-((E)-(4-((E)-(4- sulfona-tophenyl)diazenyl)phenyl)diazenyl)benzenesulfonate.
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 10.0 mg of the tested substance were dissolved in 100 ml test medium to prepare a stock solution of 0.10 mg/ml. Calculated volumes of this stock solution were diluted with test medium to obtain standard solutions in the range from 0.1 mg/1 to 25.0 mg/L of tested substance.
- Sampling method: The stock solution of the biological test was diluted l000 fold with test medium. Due to precipitation effects, samples with a nomial concentration of 309 mg/1 and 1000 mg/1 sampled test must have been filtered first. Samples with a nominal concentration of 309 mg/1 were diluted l0 fold with test medium. Samples with a nomial concentration of 1000 mg/1 were diluted 50 fold with test medium.
- Sample storage conditions before analysis: The samples were stored deep-frozen (-20° C) until the analysis was performed.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
-Common name: Daphnia magna Straus (6-24 hours old)
-Source: RCC laboratories
-The parental Daphnia were maintained in the same reconstituted water as in the test at least 24 hours prior to the start of the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/l as CaCCO3
- Test temperature:
- 19.5-19.8°C
- pH:
- 7.9-8.7
- Dissolved oxygen:
- >8.2 mg/l
- Salinity:
- CaCl2 x 2H20 294 mg/l
MgS04 x 7H20 123 mg/l
NaHC03 65 mg/l
KCl 5.8 mg/l - Nominal and measured concentrations:
- A stock solution was prepared by dissolving the test substance in test water (10.0 g/1). The stock solution was diluted in a series of sequential
dilutions with test water to prepare the following final nominal concentrations: 95, 171, 309, 556 and 1000 mg test substance/l - Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: Mg= 4 : 1; Na : K = 10 : 1
- Conductivity: 0.1 microS/cm
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours/day, about 200 - 600 Lux
- Light intensity: 200-600 lux - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Up to the highest nominal concentration tested (1000 mg/L), no toxic effect on Daphnia magna was observed. Therefore, the NOEC (highest concentration tested without toxic effect) of the tested sample after 48 hours test duration is 1000 mg/L. The substance is acutely not harmful to aquatic invertebrates.
- Executive summary:
The objective of the 48-hour toxicity test was to evaluate the influence of the test substance on the mobility respectively survival of Daphnia magna. The nominal concentrations tested were 95, 171, 309, 556 and 1000 mg test substance /L and a control without any additions. Up to the higest concentration tested of 1000 mg test substance/1 no toxic effect on Daphnia magna was observed. Therefore, the NOEC (highest concentration tested without toxic effect) of the test substance after 48 hours test duration is 1000 mg/1. Since according to the OECD Guideline No. 202 concentrations exceeding 1000 mg test substance/1 should not be tested, the 48 h EC 50 could not be specified.In the analysed test media the measured concentrations of the test substance only amounted to 29.2 - 41.7 % of the nominal values. These low recovery rates were probably due to a precipitation of the test substance, which was observed in test media of all test concentrations during test duration and also in the samples taken for the analytical measurements. Since in the stock solution a concentration was measured corresponding to 102.4 % of the nominal value, the reported results are related to the nominal concentrations of the test substance.
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