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EC number: 270-616-4 | CAS number: 68459-67-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Mar - 01 Apr 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (no purity, no data on pH and dissolved oxygen)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 1992
- Deviations:
- yes
- Remarks:
- No data on pH and dissolved oxygen is reported
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in 20 L of tap water for each test concentration. - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Source: Neil Hardy Aquatica, Surrey, UK
- Length at study initiation (length definition, mean, range and SD): 5.6 ± 0.2 cm
- Weight at study initiation (mean and range, SD): 1.94 ± 0.17 g
ACCLIMATION
- Health during acclimation (any mortality observed): < 1% in the 7 days prior to start of test, no medication - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- approx. 100 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- no data
- Dissolved oxygen:
- no data
- Nominal and measured concentrations:
- Nominal: 0, 100, 180, 320, 560 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 L
- Aeration: via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate): Daily renewal of test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.97 bodyweight/L; corpulence factor K = 1.1 g/cm3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (by the addition of Sodium thiosulphate)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities and behaviour were recorded at 3, 6, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes, but no details given. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL = 100 - 180 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: no mortalities
- The results of the test solution analysis showed an increasing carbon concentration with increasing nominal test concentration and that no significant change in carbon concentration was observed over the 96 h exposure period; the results were taken to demonstrate the stability of the test concentrations employed during the period of exposure. - Sublethal observations / clinical signs:
Table 1: Additional information on effect concentrations
Time
Endpoint
Effect conc.
Nom./Meas.
Conc. based on
Basis for effect
Remarks
3 h
LC50
> 1000 mg/L
nominal
test mat.
mortality
-
6 h
LC50
800 mg/L
nominal
test mat.
mortality
95% CL = 710 – 900 mg/L
24 h
LC50
300 mg/L
nominal
test mat.
mortality
95% CL = 250 – 360 mg/L
48 h
LC50
130 mg/L
nominal
test mat.
mortality
95% CL = 100 – 180 mg/L
72 h
LC50
130 mg/L
nominal
test mat.
mortality
95% CL = 100 – 180 mg/L
96 h
LC50
130 mg/L
nominal
test mat.
mortality
95% CL = 100 – 180 mg/L
Highest test concentration resulting in 0% mortality = 100 mg/L
Lowest test concentration resulting in 100% mortality = 180 mg/L
Table 2: Cumulative Mortality Data for Golden Orfe Exposed for 96 h to the test substance
Nominal Concentration of test substance (mg/L)
Cumulative Mortality
(initial population: 10)
3 h
6 h
24 h
48 h
72 h
96 h
Control
0
0
0
0
0
0
100
0
0
0
0
0
0
180
0
0
0
10
10
10
320
0
0
6
10
10
10
560
0
0
10
10
10
10
1000
0
8
10
10
10
10
Table 3: Marked reactions other than death
Abnormality
Test Concentration
Time
Number of Fish
Loss of equilibrium
1000 mg/L
3 h
10/10
560 mg/L
6 h
7/10
Presence of moribund fish
1000 mg/L
6 h
2/2
560 mg/L
6 h
3/10
Lethargy
320 mg/L
24 h
4/4
Table 4: Results from Total Organic Carbon (TOC) Analysis
Samples
Nominal Concentration (mg/L)
Concentration of TOC corrected for control (mg C/L)
0 h (fresh media)
100
60.07
180
97.19
320
187.19
560
331.79
1000
572.89
24 h (old media)
100
51.258
180
84.198
320
162.138
560
259.538
1000
497.538
72 h (fresh media)
100
52.031
96 h (old media)
100
50.147
Reference
Description of key information
The study was conducted according to OECD 203.
LC50 (96h) = 130 mg/L
NOEC (96h) = 100 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 130 mg/L
Additional information
The study with Castor oil, ester with glycerol (CAS 68459-67-6) was performed according to OECD 203 (GLP compliance not specified) under semi-static conditions using the freshwater species Leuciscus idus as test organism. The fish were exposed to nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L over a period of 96h.. The highest test concentration resulting in 0% mortality was 100 mg/L. The lowest test concentration resulting in 100% mortality was 180 mg/L. The LC50 (96h) was determined to be 130 mg/L.
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