Butyl(1-methylpropyl)magnesium

Short-term toxicity to fish

Description of key information

The test substance dibutylmagnesium was examined in a acute toxicity test for fish according OECD Guideline 203. The result indicated that the LC50(96h)  was greater than 100 mg/L (highest nominal observed test concentration). 

Additional information

This study was conducted to determine the acute toxicity of the test substance Dibutylmagnesium to Brachydanio rerio after 96 h test period. Result of preliminary test indicated that there was no dead fish observed and the fish had no abnormal behaviour during the test period under nominal concentrations of 1, 10 and 100 mg/L. Therefore it was not necessary to conduct the definitive test. However limit test with 100 mg/L test solution was carried out for 3 replicates. Each replicate consists of 10 organisms in 5 L aquarium. The results indicated after 96 h exposure, that there was no dead fish and no abnormal behaviour observed during

test period. This demonstrated that the LC50 (96h) of Methylmagnesium chloride for fish was greater than highest tested nominal concentration of 100 mg/L, which is equivalent to analytical measured concentration between 36.2 mg/L to 40.65 mg/L (determined with atomic absorption spectrometry). Potassium dichromate was used as positive control. The calculated LC50 (24h) = 213.67 - 288.34 mg/L is in accordance with the accepted 200 - 400 mg/L range which satisfied the test requirement.