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EC number: 251-974-0 | CAS number: 34375-28-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance 2-[hydroxy(methyl)amino]ethanol.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2-(hydroxymethylamino)ethanol
- Molecular formula (if other than submission substance): C3H9NO2
- Molecular weight (if other than submission substance): 91.1091 g/mol
- Smiles notation (if other than submission substance): CN(CCO)O
- InChl (if other than submission substance): 1S/C3H9NO2/c1-4(6)2-3-5/h5-6H,2-3H2,1H3
- Substance type: Organic
- Physical state: Liquid - Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Concentration of sludge: 30mg/l
- Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD
- Key result
- Parameter:
- other: BOD
- Value:
- 68
- Sampling time:
- 14 d
- Remarks on result:
- other: Other details not known
- Details on results:
- Test substance undergoes 68% degradation by BOD parameter in 14 days.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance2-[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
- Executive summary:
Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance 2 -[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance 2 -[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
Reference
Description of key information
Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance 2 -[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5) (EnviChem, 2014). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance 2 -[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Various experimental and predicted data for the target compound 2 -[hydroxy(methyl)amino]ethanol (CAS No. 34375 -28 -5) and various supporting weight of evidence studies for its read across substances were reviewed for the biodegradation end point which are summarized as below:
In an experimental study from secondary source (EnviChem, 2014), biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of test substance 2 -[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 68% degradation by BOD parameter in 14 days. Thus, based on percentage degradation, test substance 2 -[hydroxy(methyl)amino]ethanol was considered to be readily biodegradable in nature.
In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound 2-[hydroxy(methyl)amino]ethanol(CAS No. 34375-28-5) in the presence of mixed populations of environmental microorganisms was estimated.The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI LInear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 2 -[hydroxy(methyl) amino]ethanol is expected to be readily biodegradable.
In a supporting weight of evidence study from authoritative database and secondary source (J-CHECK and OECD SIDS) for the read across chemical 1,4 -Butanediol (CAS no. 110 -63 -4),biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of read across substance 1,4 -Butanediol. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined to be 83, 94 and 100% by BOD, TOC removal and GC parameter in 14 days. Thus, based on percentage degradation,1,4-Butanediol is considered to be readily biodegradable in nature.
For the read chemical hexane (CAS no. 110-54-3) from authoritative database (HSDB, 2016),biodegradation experiment was conducted for evaluating the percentage biodegradability of read across substance hexane (CAS no. 110-54-3) under aerobic conditions. Activated sludge was used as a test inoculum for the study. The percentage degradation of read across substance was determined to be 100% in less than 30 days. Thus, based on percentage degradation, hexane is considered to be readily biodegradable in nature.
Another biodegradation study was conducted for evaluating the percentage biodegradability of read across substance heptane (CAS no. 142-82-5) under aerobic conditions (from peer reviewed journal F. Solano-Serena et. al, 1999 and authoritative database HSDB, 2016). Activated sludge was used as a test inoculum obtained from urban waste water treatment plant. A microbial suspension containing 3 g/l dry weight was obtained by centrifugation of the sludge at 15000 g for 20 mins and resuspension of the centrifuged biomass in the same volume of nutrient solution. Inoculum conc. of 100 mg/l was used. The centrifuged biomass could be used immediately or stored at -80°C for several months without significant loss of degradation capacity. The vitamin – supplemented mineral salt medium was used as a nutrient solution. Individual hydrocarbons were added to the nutrient solution as sole carbon source. 500 ml flasks closed with Teflon-coated stoppers and with side-arms equipped with Mininert valves was used as a culture apparatus for the study. Test substance was added to 50 ml of inoculated culture medium through a valve with a Hamilton syringe. After an incubation period of 25 days at 30°C with an alternative shaking (70 strokes /min), 5 ml of CH2Cl2, containing 600 mg/ml dodecane as internal standard, was introduced into the flask through the valve and the remaining hydrocarbon were extracted for 1 h under shaking. The flasks were refrigerated overnight at 4°C before opening and the suspensions were centrifuged at 35000 g for 30 min at 4°C. The CH2Cl2 phase of each flask was then analyzed by gas chromatography. Abiotic controls containing 1 g/l HgCl2 were run under the similar test conditions. Carbon dioxide was measured with a chromatograph equipped with a thermal conductivity detector and a Porapak Q column (80/100 mesh, 2 m) using an external standard method. The carried gas was helium and the column temperature was 50°C. The temperature of the injector and that of the detector was 100°C. Samples (250µl) of the headspace gas of culture flasks were withdrawn with a gas-tight syringe and injected into the chromatograph for CO2 determination. The kinetics of O2 consumption during gasoline degradation were determined in duplicate at 30°C over 25 d with the electrolytic respirometric equipment D-12 Sapromat. The percentage degradation of read across substance was determined to be 17 and 100% by O2 consumption, CO2 production and GC parameter after 2 and 25 days, respectively. Thus, based on percentage degradation, heptane is considered to be readily biodegradable in nature.
On the basis of above results for target chemical 2-[hydroxy(methyl)amino]ethanol (from EnviChem, 2014 and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK, HSDB, peer reviewed journal and secondary source OECD SIDS), it can be concluded that the test substance 2 -[hydroxy(methyl)amino]ethanol can be expected to be readily biodegradable in nature.
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